Implications of a multiscale structure of the yeast nuclear pore complex.
AlphaFold2 modeling
FG repeats
computed structure models
cryo-EM
cryogenic electron microscopy
nuclear pore complex
nucleocytoplasmic transport
single-particle analysis
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
21 09 2023
21 09 2023
Historique:
received:
03
04
2023
revised:
23
06
2023
accepted:
24
08
2023
pmc-release:
21
09
2024
medline:
25
9
2023
pubmed:
23
9
2023
entrez:
22
9
2023
Statut:
ppublish
Résumé
Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution.
Identifiants
pubmed: 37738963
pii: S1097-2765(23)00661-5
doi: 10.1016/j.molcel.2023.08.025
pmc: PMC10630966
mid: NIHMS1932079
pii:
doi:
Substances chimiques
Membrane Transport Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3283-3302.e5Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM083960
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM112108
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM117212
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM045377
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM109824
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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