Creating Polyploid Escherichia Coli and Its Application in Efficient L-Threonine Production.
L-threonine production
cell division
metabolic engineering
polyploid Escherichia coli
synthetic biology
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
11 2023
11 2023
Historique:
revised:
31
07
2023
received:
17
04
2023
medline:
6
11
2023
pubmed:
26
9
2023
entrez:
26
9
2023
Statut:
ppublish
Résumé
Prokaryotic genomes are generally organized in haploid. In synthetic biological research, efficient chassis cells must be constructed to produce bio-based products. Here, the essential division of the ftsZ gene to create functional polyploid E. coli is regulated. The artificial polyploid E. coli containing 2-4 chromosomes is confirmed through PCR amplification, terminator localization, and flow cytometry. The polyploid E. coli exhibits a larger cell size, and its low pH tolerance and acetate resistance are stronger than those of haploid E. coli. Transcriptome analysis shows that the genes of the cell's main functional pathways are significantly upregulated in the polyploid E. coli. These advantages of the polyploid E. coli results in the highest reported L-threonine yield (160.3 g L
Identifiants
pubmed: 37749873
doi: 10.1002/advs.202302417
pmc: PMC10625114
doi:
Substances chimiques
Threonine
2ZD004190S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2302417Subventions
Organisme : National Natural Science Foundation of China
ID : 31971336
Organisme : National Natural Science Foundation of China
ID : 31770095
Organisme : National Key Research and Development Program of China
ID : 2022YFC3401300
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Metab Eng. 2020 Mar;58:17-34
pubmed: 30940506
Metab Eng. 2019 Jan;51:79-87
pubmed: 30102971
Cold Spring Harb Symp Quant Biol. 1968;33:677-93
pubmed: 4892005
Science. 2009 Apr 10;324(5924):255-8
pubmed: 19359587
Sci Rep. 2017 Apr 18;7:45303
pubmed: 28417974
ACS Synth Biol. 2022 Feb 18;11(2):538-546
pubmed: 35044170
Curr Biol. 2014 Sep 22;24(18):2149-2155
pubmed: 25176632
Trends Biotechnol. 2022 Aug;40(8):918-931
pubmed: 35120750
Cell Rep. 2022 Mar 22;38(12):110539
pubmed: 35320717
Curr Opin Biotechnol. 2014 Aug;28:96-102
pubmed: 24495512
J Mol Microbiol Biotechnol. 2014;24(5-6):409-19
pubmed: 25732342
J Bacteriol. 2007 Dec;189(23):8693-703
pubmed: 17905987
Adv Sci (Weinh). 2023 Mar;10(7):e2205855
pubmed: 36642845
Curr Biol. 2015 May 4;25(9):R353-8
pubmed: 25942544
Biotechnol Adv. 2022 Mar-Apr;55:107912
pubmed: 35041862
Nat Commun. 2020 May 8;11(1):2262
pubmed: 32385264
Metab Eng. 2018 Nov;50:16-46
pubmed: 29689382
Biotechnol Adv. 2011 Jan-Feb;29(1):11-23
pubmed: 20688145
Cell. 1985 Oct;42(3):941-9
pubmed: 2996784
Crit Rev Biochem Mol Biol. 2012 May-Jun;47(3):236-49
pubmed: 22313414
Metab Eng. 2020 May;59:36-43
pubmed: 31954846
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2765-70
pubmed: 21282646
Ann N Y Acad Sci. 2013 Jan;1277:8-28
pubmed: 23215820
Trends Cell Biol. 2020 Sep;30(9):688-694
pubmed: 32646579
Mol Microbiol. 2012 Nov;86(4):770-86
pubmed: 22995042
Metab Eng. 2016 Nov;38:358-369
pubmed: 27693319
Bioresour Technol. 2023 Mar;371:128586
pubmed: 36621693
Nat Commun. 2020 Apr 2;11(1):1641
pubmed: 32242019
Mol Microbiol. 2002 Nov;46(4):985-96
pubmed: 12421305
Appl Microbiol Biotechnol. 2016 Nov;100(21):8985-9001
pubmed: 27645299
Bioprocess Biosyst Eng. 2018 Oct;41(10):1509-1518
pubmed: 30062600
Metab Eng. 2011 Mar;13(2):214-24
pubmed: 21163359
Curr Opin Cell Biol. 2021 Feb;68:163-172
pubmed: 33220539
Annu Rev Biophys. 2015;44:123-42
pubmed: 25747591
Nat Rev Genet. 2005 Nov;6(11):836-46
pubmed: 16304599
Nat Rev Genet. 2017 Jul;18(7):411-424
pubmed: 28502977
Mol Microbiol. 2013 Oct;90(2):214-27
pubmed: 23962352
Biochem Cell Biol. 2010 Apr;88(2):301-14
pubmed: 20453931
Mol Microbiol. 2010 Nov;78(4):866-82
pubmed: 20807205
Mol Microbiol. 2014 Sep;93(5):911-27
pubmed: 24995530
J Pharm Biomed Anal. 2017 May 30;139:263-268
pubmed: 28069351
Langmuir. 2022 Apr 19;38(15):4578-4588
pubmed: 35380840
Curr Biol. 2013 Nov 4;23(21):2151-6
pubmed: 24139735
J Cell Biol. 2015 May 25;209(4):485-91
pubmed: 26008741
Adv Sci (Weinh). 2023 Nov;10(31):e2302417
pubmed: 37749873
PLoS One. 2006 Dec 20;1:e92
pubmed: 17183724
J Bacteriol. 2019 Jun 10;201(13):
pubmed: 30988035
J Bacteriol. 2016 Jul 28;198(16):2166-79
pubmed: 27246569
Mol Microbiol. 2002 Oct;46(1):63-74
pubmed: 12366831
Elife. 2019 Nov 11;8:
pubmed: 31710292
Mol Syst Biol. 2007;3:149
pubmed: 18059444
Int J Mol Sci. 2022 Dec 05;23(23):
pubmed: 36499674
Mol Microbiol. 2012 Dec;86(6):1318-33
pubmed: 23078205
Nat Commun. 2021 Apr 27;12(1):2448
pubmed: 33907196
Mol Microbiol. 1998 Aug;29(4):1053-63
pubmed: 9767573
J Microbiol Methods. 2010 Sep;82(3):330-3
pubmed: 20619304
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Chem Soc Rev. 2014;43(20):6954-81
pubmed: 25017039