Modification of the Properties of Extracellular Matrix of Senescent Mesenchymal Stem Cells.

Endothelial Cells Cellular Senescence Cell Proliferation Intercellular Signaling Peptides and Proteins / pharmacology Mesenchymal Stem Cells Extracellular Matrix / metabolism Cells, Cultured Cell Differentiation

cell senescence endothelial cells extracellular matrix multipotent mesenchymal stem cells

Journal

Bulletin of experimental biology and medicine

ISSN: 1573-8221

Titre abrégé: Bull Exp Biol Med

Pays: United States

ID NLM: 0372557

Informations de publication

Date de publication:
Aug 2023

Historique:

received: 04 04 2023

medline: 9 10 2023

pubmed: 29 9 2023

entrez: 28 9 2023

Statut: ppublish

Résumé

Cell senescence leads to changes in the secretory activity of mesenchymal stem cells (MSC), including proteins of extracellular matrix (ECM). Here we studied the regulatory properties of ECM of senescent MSC in a model with endothelial cells (EC). EC were seeded onto a decellularized extracellular matrix of senescent MSC. Changes in cell morphology and a decrease in cell growth were observed. In addition, increased production of inflammatory chemokines MCP-1 and GROα and reduced synthesis of proangiogenic growth factor FGF-2 were revealed. Analysis of ECM showed quantitative and qualitative changes, including fibronectin layer morphology, total protein content, and concentration of deposited growth factors such as VEGF. Thus, our work demonstrates that senescence of MSC can lead to modification of the effects of their ECM on EC activity.

Identifiants

DOI: 10.1007/s10517-023-05905-z PMID: 37770790

pubmed: 37770790

doi: 10.1007/s10517-023-05905-z

pii: 10.1007/s10517-023-05905-z

doi:

Substances chimiques

Intercellular Signaling Peptides and Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

569-575

Informations de copyright

Références

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Modification of the Properties of Extracellular Matrix of Senescent Mesenchymal Stem Cells.

Journal

Informations de publication

Résumé

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Références

Auteurs

D K Matveeva (DK)

M I Ezdakova (MI)

A Yu Ratushnyy (AY)

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