Plant growth-promoting rhizobacterium Bacillus cereus AR156 induced systemic resistance against multiple pathogens by priming of camalexin synthesis.
Arabidopsis
Bacillus cereus (AR156)
biocontrol
camalexin
induced systemic resistance (ISR)
transcription factor
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
revised:
04
09
2023
received:
30
04
2023
accepted:
17
09
2023
medline:
5
12
2023
pubmed:
30
9
2023
entrez:
29
9
2023
Statut:
ppublish
Résumé
Phytoalexins play a crucial role in plant immunity. However, the mechanism of how phytoalexin is primed by beneficial microorganisms against broad-spectrum pathogens remains elusive. This study showed that Bacillus cereus AR156 could trigger ISR against broad-spectrum disease. RNA-sequencing and camalexin content assays showed that AR156-triggered ISR can prime the accumulation of camalexin synthesis and secretion-related genes. Moreover, it was found that AR156-triggered ISR elevates camalexin accumulation by increasing the expression of camalexin synthesis genes upon pathogen infection. We observed that the priming of camalexin accumulation by AR156 was abolished in cyp71a13 and pad3 mutants. Further investigations reveal that in the wrky33 mutant, the ability of AR156 to prime camalexin accumulation is abolished, and the mediated ISR against the three pathogens is significantly compromised. Furthermore, PEN3 and PDR12, acting as camalexin transporters, participate in AR156-induced ISR against broad-spectrum pathogens differently. In addition, salicylic acid and JA/ET signalling pathways participate in AR156-primed camalexin synthesis to resist pathogens in different forms depending on the pathogen. In summary, B. cereus AR156 triggers ISR against Botrytis cinerea, Pst DC3000 and Phytophthora capsici by priming camalexin synthesis. Our study provides deeper insights into the significant role of camalexin for AR156-induced ISR against broad-spectrum pathogens.
Substances chimiques
camalexin
0
Phytoalexins
0
Arabidopsis Proteins
0
PDR12 protein, Arabidopsis
0
ATP-Binding Cassette Transporters
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
337-353Subventions
Organisme : National Natural Science Foundation of China
ID : 32272617
Organisme : National Natural Science Foundation of China
ID : 31972322
Organisme : National Natural Science Foundation of China
ID : 32102281
Organisme : Key Construction Laboratory of Probiotics in Jiangsu Province
ID : JSYSZJ2019003
Organisme : Science and Technology Project of Jiangsu Province
ID : BE2021364
Organisme : Science and Technology Project of Jiangsu Province
ID : BE2020408
Organisme : Fundamental Research Funds for the Central Universities
ID : KYZZ2022001
Informations de copyright
© 2023 John Wiley & Sons Ltd.
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