DeoR regulates lincomycin production in Streptomyces lincolnensis.

Lincomycin / metabolism Bacterial Proteins / genetics Streptomyces / genetics Secondary Metabolism Gene Expression Regulation, Bacterial
DeoR family Lincomycin Streptomyces lincolnensis Transcriptional regulation

Journal

World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472

Informations de publication

Date de publication:
06 Oct 2023
Historique:
received: 14 07 2023
accepted: 02 10 2023
medline: 2 11 2023
pubmed: 6 10 2023
entrez: 6 10 2023
Statut: epublish

Résumé

Regulators belonging to the DeoR family are widely distributed among the bacteria. Few studies have reported that DeoR family proteins regulate secondary metabolism of Streptomyces. This study explored the function of DeoR (SLINC_8027) in Streptomyces lincolnensis. Deletion of deoR in NRRL 2936 led to an increase in cell growth. The lincomycin production of the deoR deleted strain ΔdeoR was 3.4-fold higher than that of the wild strain. This trait can be recovered to a certain extent in the deoR complemented strain ΔdeoR::pdeoR. According to qRT-PCR analysis, DeoR inhibited the transcription of all detectable genes in the lincomycin biosynthesis cluster and repressed the expression of glnR, bldD, and SLCG_Lrp, which encode regulators outside the cluster. DeoR also inhibited the transcription of itself, as revealed by the XylE reporter. Furthermore, we demonstrated that DeoR bound directly to the promoter region of deoR, lmbA, lmbC-D, lmbJ-K, lmrA, lmrC, glnR, and SLCG_Lrp, by recognizing the 5'-CGATCR-3' motif. This study found that versatile regulatory factor DeoR negatively regulates lincomycin biosynthesis and cellular growth in S. lincolnensis, which expanded the regulatory network of lincomycin biosynthesis.

Identifiants

DOI: 10.1007/s11274-023-03788-w PMID: 37801155
pubmed: 37801155
doi: 10.1007/s11274-023-03788-w
pii: 10.1007/s11274-023-03788-w
doi:

Substances chimiques

Lincomycin BOD072YW0F
Bacterial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

332

Subventions

Organisme : National Natural Science Foundation of China
ID : 31900059
Organisme : Ministry of Science and Technology of the People's Republic of China
ID : 2021YFC2100600

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Jingyun Zou (J)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.

Yue Mao (Y)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.

Bingbing Hou (B)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.

Yajing Kang (Y)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.

Ruida Wang (R)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. biord726@163.com.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China. biord726@163.com.

Haizhen Wu (H)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. wuhzh@ecust.edu.cn.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China. wuhzh@ecust.edu.cn.

Jiang Ye (J)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.

Huizhan Zhang (H)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Department of Applied Biology, East China University of Science and Technology, Shanghai, 200237, China.

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Classifications MeSH

questionsmedicales.fr › Glucides › Hétérosides › Lincosamides › Lincomycine › DeoR regulates lincomycin production in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › DeoR regulates lincomycin production in...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › DeoR regulates lincomycin production in...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Métabolisme secondaire › DeoR regulates lincomycin production in...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › DeoR regulates lincomycin production in...