Relay run property of immobilized cholesterol oxidase on magnetic biochar for sterol removal.
Fenton process
Immobilized enzyme
Magnetic biochar
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
31 Dec 2023
31 Dec 2023
Historique:
received:
03
06
2023
revised:
05
09
2023
accepted:
03
10
2023
medline:
23
11
2023
pubmed:
7
10
2023
entrez:
6
10
2023
Statut:
ppublish
Résumé
This study investigated the biocatalytic performance of immobilized cholesterol oxidase (CHOD) on magnetite-based carbon (MBC) for degrading cholesterol. The results showed that MBC-CHOD exhibited higher activity and good affinity towards substrate compared to free enzyme and other immobilized enzymes. Mass spectra analysis revealed that MBC-CHOD damaged the main structure of cholesterol, benefitting the further biological treatment. The study proposes a Fenton process mechanism by which H
Identifiants
pubmed: 37802448
pii: S0141-8130(23)04147-8
doi: 10.1016/j.ijbiomac.2023.127250
pii:
doi:
Substances chimiques
biochar
0
Sterols
0
Cholesterol Oxidase
EC 1.1.3.6
Hydrogen Peroxide
BBX060AN9V
Charcoal
16291-96-6
Carbon
7440-44-0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127250Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.