Virulence potential of Salmonella 1,4, [5],12:i:- strains isolated during decades from different sources in the Southeast region of Brazil.
Acid stress
Galleria mellonella
Monophasic Salmonella
Oxidative stress
Virulence genes
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
01
06
2023
accepted:
30
09
2023
pmc-release:
10
10
2024
medline:
4
12
2023
pubmed:
11
10
2023
entrez:
10
10
2023
Statut:
ppublish
Résumé
Salmonella 1,4, [5],12:i:- is one of the most prevalent serovars associated with gastroenteritis in several countries, including Brazil. However, few studies have analyzed the virulence potential of this variant in this country. Therefore, this study aimed to characterize S. 1,4, [5],12:i:- strains isolated in Southeast Brazil. To this end, 113 S. 1,4, [5],12:i:- strains isolated from different sources between 1983 and 2020 were analyzed. For all strains, the frequencies of 11 virulence genes were investigated using PCR and the molecular typing was performed using pulsed-field gel electrophoresis (PFGE). Furthermore, 40 strains isolated from human and non-human sources were characterized by survival under acid and oxidative stress, and virulence analysis in Galleria mellonella was performed for 20 selected strains. All virulence genes were detected in more than 91% of the strains. The studied strains were grouped into four clusters using PFGE. Most strains were present in one cluster, named PFGE-A, with a genetic similarity of ≥ 79.5%. All 40 strains survived acid stress after 10 min and 1 h of exposure. Under oxidative stress, all 40 strains survived after 10 min, and 36 survived after 1 h of exposure. In the G. mellonella assay, nine isolates from non-human sources and six isolates from human showed high-to-intermediate virulence profiles. In conclusion, the pathogenic potential of the strains studied was corroborated by the high frequency of all the virulence genes identified. The PFGE results suggested that most strains belonged to one main cluster that has been prevailing in the São Paulo State, Brazil. The S. 1,4, [5],12:i:- strains isolated from human and non-human sources successfully survived the unfavorable conditions in the human gastrointestinal tract. Finally, strains isolated from non-human sources showed a higher proportion of isolates with high to intermediate virulence profiles in G. mellonella than in human isolates, suggesting a possible difference between isolates from different origins.
Identifiants
pubmed: 37817050
doi: 10.1007/s42770-023-01145-5
pii: 10.1007/s42770-023-01145-5
pmc: PMC10689702
doi:
Substances chimiques
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2827-2843Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2022/07013-0
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2019/19338-8
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 88887.523672/2020-00
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 304399/2018-3
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 304803/2021-9
Informations de copyright
© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
Références
Biol Res. 2022 Feb 22;55(1):8
pubmed: 35193678
Infect Genet Evol. 2013 Oct;19:145-51
pubmed: 23860124
Microbiology (Reading). 2011 May;157(Pt 5):1428-1445
pubmed: 21292746
Can J Vet Res. 2011 Jan;75(1):49-56
pubmed: 21461195
Sci Total Environ. 2020 Feb 10;703:134609
pubmed: 31753504
Prev Vet Med. 2020 Jun;179:105024
pubmed: 32417637
Epidemiol Infect. 2014 Jul;142(7):1403-10
pubmed: 24625654
Res Microbiol. 2011 Feb-Mar;162(2):214-22
pubmed: 21144897
J Vet Med Sci. 2018 Dec 11;80(12):1839-1846
pubmed: 30369553
J Clin Microbiol. 1988 Nov;26(11):2465-6
pubmed: 3069867
Foodborne Pathog Dis. 2021 Nov;18(11):790-797
pubmed: 34287022
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11978-82
pubmed: 1465428
Foods. 2021 Jul 27;10(8):
pubmed: 34441509
Lancet Infect Dis. 2018 Nov;18(11):1211-1228
pubmed: 30243583
Foodborne Pathog Dis. 2020 Feb;17(2):87-97
pubmed: 31532231
Ital J Food Saf. 2018 Jul 03;7(2):7223
pubmed: 30046559
Food Sci Nutr. 2021 Jul 16;9(9):4701-4710
pubmed: 34531984
Foodborne Pathog Dis. 2009 Jul-Aug;6(6):711-7
pubmed: 19580448
BMC Microbiol. 2012 May 22;12:63
pubmed: 22545862
Antibiotics (Basel). 2022 Apr 16;11(4):
pubmed: 35453283
Genes (Basel). 2020 Sep 01;11(9):
pubmed: 32883017
Virulence. 2018 Jan 1;9(1):281-286
pubmed: 28102761
Foods. 2021 Jul 28;10(8):
pubmed: 34441520
PLoS One. 2018 Sep 11;13(9):e0203698
pubmed: 30204776
PLoS One. 2022 May 19;17(5):e0266443
pubmed: 35588421
FEBS J. 2022 Jul;289(14):4192-4211
pubmed: 34546626
Mycopathologia. 2006 Jun;161(6):377-84
pubmed: 16761185
Infect Genet Evol. 2020 Jun;80:104193
pubmed: 31931258
Nat Commun. 2021 Feb 9;12(1):879
pubmed: 33563986
BMC Microbiol. 2021 Dec 11;21(1):338
pubmed: 34895140
J Med Microbiol. 2006 Nov;55(Pt 11):1539-1548
pubmed: 17030914
Foods. 2021 Apr 21;10(5):
pubmed: 33919142
Pathogens. 2020 Aug 26;9(9):
pubmed: 32859122
PLoS One. 2020 Aug 18;15(8):e0237886
pubmed: 32810191
Foodborne Pathog Dis. 2016 Nov;13(11):633-641
pubmed: 27768382
Foodborne Pathog Dis. 2009 Nov;6(9):1147-55
pubmed: 19694554
Foodborne Pathog Dis. 2006 Spring;3(1):59-67
pubmed: 16602980
Microbiol Spectr. 2021 Sep 3;9(1):e0024821
pubmed: 34346743
J Clin Microbiol. 1999 Jun;37(6):1661-9
pubmed: 10325304
Genes (Basel). 2021 Mar 25;12(4):
pubmed: 33806186
J Microbiol. 2021 Sep;59(9):861-870
pubmed: 34382146
Infect Genet Evol. 2021 Sep;93:104977
pubmed: 34174480
Front Cell Infect Microbiol. 2019 Jul 31;9:270
pubmed: 31428589
Rev Inst Med Trop Sao Paulo. 2022 Sep 30;64:e66
pubmed: 36197427
J Med Microbiol. 2015 Nov;64(11):1395-1407
pubmed: 26307078
Antibiotics (Basel). 2021 Feb 13;10(2):
pubmed: 33668483
Microbiol Spectr. 2021 Dec 22;9(3):e0151921
pubmed: 34851152
J Fungi (Basel). 2018 Nov 27;4(4):
pubmed: 30486393
Microorganisms. 2021 Apr 27;9(5):
pubmed: 33925758