Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures.
DNA Nanostructures
Restriction Enzymes
Sequential Brush Growth
Surface-Initiated Polymerization
Terminal Deoxynucleotidyl Transferase
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
received:
24
08
2023
medline:
22
11
2023
pubmed:
11
10
2023
entrez:
11
10
2023
Statut:
ppublish
Résumé
DNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these structures is severely compromised in biological milieu due to their fast degradation by nucleases. Recently, we showed how enzymatic polymerization could be harnessed to grow polynucleotide brushes of tunable length and location on the surface of DNA origami nanostructures, which greatly enhances their nuclease stability. Here, we report on strategies that allow for both spatial and temporal control over polymerization through activatable initiation, cleavage, and regeneration of polynucleotide brushes using restriction enzymes. The ability to site-specifically decorate DNA origami nanostructures with polynucleotide brushes in a spatiotemporally controlled way provides access to "smart" functionalized DNA architectures with potential applications in drug delivery and supramolecular assembly.
Identifiants
pubmed: 37820028
doi: 10.1002/anie.202311727
doi:
Substances chimiques
Polynucleotides
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202311727Subventions
Organisme : NIBIB NIH HHS
ID : R21 EB026590-03
Pays : United States
Organisme : NIBIB NIH HHS
ID : R21 EB026590-03
Pays : United States
Informations de copyright
© 2023 Wiley-VCH GmbH.
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