A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 10 2023
23 10 2023
Historique:
received:
15
06
2023
accepted:
13
10
2023
medline:
27
10
2023
pubmed:
24
10
2023
entrez:
23
10
2023
Statut:
epublish
Résumé
Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (ORC). In yeast, ORC is a dynamic protein that diffuses rapidly along DNA, unless halted by origin recognition sequences. However, less is known about the dynamics of ORC proteins in the presence of nucleosomes and attendant consequences for MCM loading. To address this, we harnessed an in vitro single-molecule approach to interrogate a chromatinized origin of replication. We find that ORC binds the origin of replication with similar efficiency independently of whether the origin is chromatinized, despite ORC mobility being reduced by the presence of nucleosomes. Recruitment of MCM also proceeds efficiently on a chromatinized origin, but subsequent movement of MCM away from the origin is severely constrained. These findings suggest that chromatinized origins in yeast are essential for the local retention of MCM, which may facilitate subsequent assembly of the replisome.
Identifiants
pubmed: 37872142
doi: 10.1038/s41467-023-42524-8
pii: 10.1038/s41467-023-42524-8
pmc: PMC10593741
doi:
Substances chimiques
Origin Recognition Complex
0
Nucleosomes
0
Cell Cycle Proteins
0
DNA
9007-49-2
Minichromosome Maintenance Proteins
EC 3.6.4.12
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6735Informations de copyright
© 2023. Springer Nature Limited.
Références
Nat Protoc. 2013 Mar;8(3):525-38
pubmed: 23411634
Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2206513119
pubmed: 35939666
Mol Cell. 2017 Jan 5;65(1):117-130
pubmed: 27989438
Mol Cell. 2017 Jan 5;65(1):105-116
pubmed: 27989442
Science. 2017 Jul 21;357(6348):314-318
pubmed: 28729513
Science. 1994 Sep 9;265(5178):1599-600
pubmed: 8079175
Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Oct;82(4 Pt 1):041914
pubmed: 21230320
Science. 2018 Sep 28;361(6409):1389-1392
pubmed: 30115746
Nat Commun. 2019 Jul 1;10(1):2894
pubmed: 31263106
Genome Res. 2013 Apr;23(4):698-704
pubmed: 23241746
Nature. 2013 Mar 21;495(7441):339-43
pubmed: 23474987
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1960-5
pubmed: 11854495
Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20240-5
pubmed: 19910535
Nat Commun. 2013;4:2579
pubmed: 24113677
Nature. 2023 Apr;616(7958):836-842
pubmed: 37020028
Science. 1992 Feb 14;255(5046):817-23
pubmed: 1536007
Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15090-5
pubmed: 25288749
J Biol Chem. 2007 Apr 20;282(16):11705-14
pubmed: 17314092
Nat Methods. 2020 Mar;17(3):352
pubmed: 32094914
Methods Enzymol. 2004;375:23-44
pubmed: 14870657
Nature. 1992 May 14;357(6374):128-34
pubmed: 1579162
Genetics. 2016 Jul;203(3):1027-67
pubmed: 27384026
Philos Trans R Soc Lond B Biol Sci. 2017 Oct 5;372(1731):
pubmed: 28847824
Cell. 2009 Nov 13;139(4):719-30
pubmed: 19896182
Nature. 2019 Nov;575(7784):704-710
pubmed: 31748745
Nature. 2015 Mar 26;519(7544):431-5
pubmed: 25739503
Nature. 2018 Jul;559(7713):217-222
pubmed: 29973722
Genes Dev. 2010 Apr 15;24(8):748-53
pubmed: 20351051
J Biol Chem. 2002 Oct 4;277(40):37456-63
pubmed: 12147705
Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15871-6
pubmed: 17043216
Biochim Biophys Acta. 1994 Nov 22;1219(3):677-89
pubmed: 7948025
Elife. 2017 Mar 21;6:
pubmed: 28322723
J Biol Chem. 2011 Feb 4;286(5):4021-6
pubmed: 21115484
Nat Commun. 2022 Aug 23;13(1):4947
pubmed: 35999198
J Mol Biol. 1998 Feb 13;276(1):19-42
pubmed: 9514715
Nucleic Acids Res. 1981 Jan 24;9(2):445-57
pubmed: 7208356
Nature. 2018 Mar 8;555(7695):265-268
pubmed: 29489749
Nat Commun. 2017 Dec 21;8(1):2241
pubmed: 29269875
Nat Commun. 2023 Apr 14;14(1):2082
pubmed: 37059705
Mol Cell. 2017 Jan 5;65(1):131-141
pubmed: 27989437
Science. 2012 Oct 5;338(6103):94-7
pubmed: 22983709
Mol Cell. 2010 Mar 26;37(6):834-42
pubmed: 20347425
Nat Commun. 2021 Mar 26;12(1):1908
pubmed: 33772005
Mol Cell. 2001 Jan;7(1):21-30
pubmed: 11172708
Nat Methods. 2008 Aug;5(8):695-702
pubmed: 18641657
Proc Natl Acad Sci U S A. 2022 Oct 18;119(42):e2211568119
pubmed: 36215487