Kinetic and structural details of urease inactivation by thiuram disulphides.

Enzyme inactivation Nickel Protein X-ray crystallography Quantum mechanical calculations Thiuram disulphides Urease

Journal

Journal of inorganic biochemistry
ISSN: 1873-3344
Titre abrégé: J Inorg Biochem
Pays: United States
ID NLM: 7905788

Informations de publication

Date de publication:
Jan 2024
Historique:
received: 08 08 2023
revised: 21 09 2023
accepted: 07 10 2023
medline: 7 12 2023
pubmed: 26 10 2023
entrez: 25 10 2023
Statut: ppublish

Résumé

This paper reports on the molecular details of the reactivity of urease, a nickel-dependent enzyme that catalyses the last step of organic nitrogen mineralization, with thiuram disulphides, a class of molecules known to inactivate the enzyme with high efficacy but for which the mechanism of action had not been yet established. IC

Identifiants

pubmed: 37879152
pii: S0162-0134(23)00280-5
doi: 10.1016/j.jinorgbio.2023.112398
pii:
doi:

Substances chimiques

Thiram 0D771IS0FH
Nickel 7OV03QG267
Urease EC 3.5.1.5
Cysteine K848JZ4886
Protons 0
Disulfiram TR3MLJ1UAI
Urea 8W8T17847W

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

112398

Informations de copyright

Copyright © 2023. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Luca Mazzei (L)

Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Viale Giuseppe Fanin 40, Bologna I-40127, Italy. Electronic address: luca.mazzei2@unibo.it.

Arundhati Paul (A)

Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Viale Giuseppe Fanin 40, Bologna I-40127, Italy.

Michele Cianci (M)

Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche 10, Ancona I-60131, Italy.

Marta Devodier (M)

Computational Biomedicine, Institute of Advanced Simulations IAS-5/Institute for Neuroscience and Medicine INM-9, Forschungszentrum Jülich GmbH, Jülich D-52428, Germany; Università degli Studi di Parma, Via Università 12, Parma I-43121, Italy.

Davide Mandelli (D)

Computational Biomedicine, Institute of Advanced Simulations IAS-5/Institute for Neuroscience and Medicine INM-9, Forschungszentrum Jülich GmbH, Jülich D-52428, Germany.

Paolo Carloni (P)

Computational Biomedicine, Institute of Advanced Simulations IAS-5/Institute for Neuroscience and Medicine INM-9, Forschungszentrum Jülich GmbH, Jülich D-52428, Germany; Department of Physics and Universitätsklinikum, RWTH Aachen University, Aachen D-52074, Germany.

Stefano Ciurli (S)

Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Viale Giuseppe Fanin 40, Bologna I-40127, Italy.

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Classifications MeSH