Diacylglycerol-dependent hexamers of the SNARE-assembling chaperone Munc13-1 cooperatively bind vesicles.
Munc13-1 clusters
diacylglycerol
membrane fusion
neurotransmission
synaptic vesicle
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
31 Oct 2023
31 Oct 2023
Historique:
medline:
30
10
2023
pubmed:
26
10
2023
entrez:
26
10
2023
Statut:
ppublish
Résumé
Munc13-1 is essential for vesicle docking and fusion at the active zone of synapses. Here, we report that Munc13-1 self-assembles into molecular clusters within diacylglycerol-rich microdomains present in phospholipid bilayers. Although the copy number of Munc13-1 molecules in these clusters has a broad distribution, a systematic Poisson analysis shows that this is most likely the result of two molecular species: monomers and mainly hexameric oligomers. Each oligomer is able to capture one vesicle independently. Hexamers have also been observed in crystals of Munc13-1 that form between opposed phospholipid bilayers [K. Grushin, R. V. Kalyana Sundaram, C. V. Sindelar, J. E. Rothman,
Identifiants
pubmed: 37883433
doi: 10.1073/pnas.2306086120
pmc: PMC10623011
doi:
Substances chimiques
SNARE Proteins
0
Diglycerides
0
Molecular Chaperones
0
Phospholipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2306086120Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK027044
Pays : United States
Organisme : NIDDK NIH HHS
ID : R37 DK027044
Pays : United States
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