On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations.
cationic lipids
computational modeling
fluorescence microscopy
fusogenic liposomes
membrane phase separation
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
30 09 2023
30 09 2023
Historique:
received:
22
08
2023
revised:
19
09
2023
accepted:
27
09
2023
medline:
30
10
2023
pubmed:
28
10
2023
entrez:
28
10
2023
Statut:
epublish
Résumé
This paper studies the fusogenicity of cationic liposomes in relation to their surface distribution of cationic lipids and utilizes membrane phase separation to control this surface distribution. It is found that concentrating the cationic lipids into small surface patches on liposomes, through phase-separation, can enhance liposome's fusogenicity. Further concentrating these lipids into smaller patches on the surface of liposomes led to an increased level of fusogenicity. These experimental findings are supported by numerical simulations using a mathematical model for phase-separated charged liposomes. Findings of this study may be used for design and development of highly fusogenic liposomes with minimal level of toxicity.
Identifiants
pubmed: 37892155
pii: biom13101473
doi: 10.3390/biom13101473
pmc: PMC10605210
pii:
doi:
Substances chimiques
Liposomes
0
Lipids
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : US National Science Foundation (NSF)
ID : DMS-1953535
Organisme : US National Science Foundation (NSF
ID : DMS-2309197
Organisme : US National Science Foundation (NSF)
ID : DMR-1753328
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