Is the Neuropeptide PEN a Ligand of GPR83?
GPCR
GPR83
PEN
orphan receptor
peptide
proSAAS
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
12 Oct 2023
12 Oct 2023
Historique:
received:
31
08
2023
revised:
03
10
2023
accepted:
05
10
2023
medline:
30
10
2023
pubmed:
28
10
2023
entrez:
28
10
2023
Statut:
epublish
Résumé
G protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second messenger and other GPCR activation assays as well as a radioligand binding assay, and using multiple GPR83 plasmids and PEN peptides from different sources, no experimental evidence was found to support a role of PEN as a GPR83 ligand.
Identifiants
pubmed: 37894796
pii: ijms242015117
doi: 10.3390/ijms242015117
pmc: PMC10606834
pii:
doi:
Substances chimiques
Ligands
0
Receptors, G-Protein-Coupled
0
Neuropeptides
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Federal Ministry of Education and Research
ID : 03IPT614A
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB1423-421152132
Organisme : Germany´s Excellence Strategy
ID : EXC2008-390540038-UniSysCat
Organisme : European Union
ID : 956314[ALLODD]
Références
PLoS One. 2011;6(12):e28152
pubmed: 22164236
J Immunol. 2006 Jul 1;177(1):209-15
pubmed: 16785516
Nature. 2020 Nov;587(7833):258-263
pubmed: 33116307
Mol Cell Biol. 2007 Dec;27(23):8065-72
pubmed: 17893329
J Biol Chem. 2006 Dec 15;281(50):38812-24
pubmed: 17015451
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):64-9
pubmed: 18165312
Immunology. 2008 Nov;125(3):302-12
pubmed: 18479351
Nat Struct Mol Biol. 2015 May;22(5):362-9
pubmed: 25895059
Biochem J. 2011 Jan 1;433(1):11-8
pubmed: 21158738
Metabolism. 2012 Oct;61(10):1486-93
pubmed: 22560055
J Med Chem. 2016 Nov 23;59(22):10100-10112
pubmed: 27791374
Prog Mol Biol Transl Sci. 2018;159:1-25
pubmed: 30340784
F S Sci. 2020 Aug;1(1):67-77
pubmed: 35559741
ACS Chem Neurosci. 2019 Apr 17;10(4):1884-1891
pubmed: 30726666
Cell. 2019 Oct 31;179(4):895-908.e21
pubmed: 31675498
Gastroenterology. 1991 Aug;101(2):303-11
pubmed: 1712329
Front Neurosci. 2021 Aug 26;15:675769
pubmed: 34512237
Clin Transl Med. 2022 Nov;12(11):e1091
pubmed: 36314066
Nat Commun. 2013;4:1968
pubmed: 23744028
J Cell Sci. 2002 Feb 1;115(Pt 3):455-65
pubmed: 11861753
FEBS J. 2023 Jul;290(13):3461-3479
pubmed: 36853120
PLoS One. 2016 Dec 9;11(12):e0168260
pubmed: 27936173
SLAS Discov. 2020 Oct;25(9):1047-1063
pubmed: 32713278
Trends Pharmacol Sci. 2010 Mar;31(3):124-30
pubmed: 20060175
PLoS One. 2013;8(1):e53347
pubmed: 23335960
Sci Signal. 2016 Apr 26;9(425):ra43
pubmed: 27117253
Biochem Biophys Res Commun. 2007 Jan 12;352(2):397-403
pubmed: 17134677
Mol Pharmacol. 2022 May 23;:
pubmed: 35605991
World J Biol Chem. 2018 Dec 12;9(3):25-35
pubmed: 30595812
J Endocr Soc. 2018 Dec 06;3(2):314-323
pubmed: 30652132