Dormant bacterial spores encrypt a long-lasting transcriptional program to be executed during revival.

Spores, Bacterial / genetics Bacillus subtilis / genetics Bacterial Proteins / genetics Sigma Factor / genetics Promoter Regions, Genetic DNA-Directed RNA Polymerases / genetics
Bacillus subtilis Csp RNAP SASP chromosome structure spore dormancy spore germination sporulation transcription

Journal

Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571

Informations de publication

Date de publication:
16 Nov 2023
Historique:
received: 19 01 2023
revised: 16 08 2023
accepted: 12 10 2023
medline: 27 11 2023
pubmed: 11 11 2023
entrez: 10 11 2023
Statut: ppublish

Résumé

Sporulating bacteria can retreat into long-lasting dormant spores that preserve the capacity to germinate when propitious. However, how the revival transcriptional program is memorized for years remains elusive. We revealed that in dormant spores, core RNA polymerase (RNAP) resides in a central chromosomal domain, where it remains bound to a subset of intergenic promoter regions. These regions regulate genes encoding for most essential cellular functions, such as rRNAs and tRNAs. Upon awakening, RNAP recruits key transcriptional components, including sigma factor, and progresses to express the adjacent downstream genes. Mutants devoid of spore DNA-compacting proteins exhibit scattered RNAP localization and subsequently disordered firing of gene expression during germination. Accordingly, we propose that the spore chromosome is structured to preserve the transcriptional program by halting RNAP, prepared to execute transcription at the auspicious time. Such a mechanism may sustain long-term transcriptional programs in diverse organisms displaying a quiescent life form.

Identifiants

DOI: 10.1016/j.molcel.2023.10.010 PMID: 37949068
pubmed: 37949068
pii: S1097-2765(23)00844-4
doi: 10.1016/j.molcel.2023.10.010
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Sigma Factor 0
DNA-Directed RNA Polymerases EC 2.7.7.6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4158-4173.e7

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Bing Zhou (B)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel.

Yifei Xiong (Y)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel.

Yuval Nevo (Y)

Info-CORE, Bioinformatics Unit of the I-CORE Computation Center at the Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Tamar Kahan (T)

Bioinformatics Unit, Faculty of Medicine, The Hebrew University of Jerusalem, 9112001 Jerusalem, Israel.

Oren Yakovian (O)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel; The Racah Institute of Physics, Faculty of Science, The Hebrew University of Jerusalem, 9190401 Jerusalem, Israel.

Sima Alon (S)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel.

Saurabh Bhattacharya (S)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel.

Ilan Rosenshine (I)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel.

Lior Sinai (L)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel. Electronic address: lior.sinai@mail.huji.ac.il.

Sigal Ben-Yehuda (S)

Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, The Hebrew University of Jerusalem, P.O.B. 12272, 9112001 Jerusalem, Israel. Electronic address: sigalb@ekmd.huji.ac.il.

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Classifications MeSH

questionsmedicales.fr › Stades d'organismes › Spores › Spores bactériens › Dormant bacterial spores encrypt a...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › Dormant bacterial spores encrypt a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Dormant bacterial spores encrypt a...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteur sigma › Dormant bacterial spores encrypt a...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Dormant bacterial spores encrypt a...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › Dormant bacterial spores encrypt a...