MitoTracker: A useful tool in need of better alternatives.

Fluorescent Dyes Mitochondria
Fluorescent probes Membrane potential MitoTracker Mitochondria

Journal

European journal of cell biology
ISSN: 1618-1298
Titre abrégé: Eur J Cell Biol
Pays: Germany
ID NLM: 7906240

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 16 08 2023
revised: 10 10 2023
accepted: 30 10 2023
medline: 24 11 2023
pubmed: 13 11 2023
entrez: 13 11 2023
Statut: ppublish

Résumé

The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.

Identifiants

DOI: 10.1016/j.ejcb.2023.151371 PMID: 37956476
pubmed: 37956476
pii: S0171-9335(23)00086-9
doi: 10.1016/j.ejcb.2023.151371
pii:
doi:

Substances chimiques

Fluorescent Dyes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

151371

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Kit Neikirk (K)

Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.

Andrea G Marshall (AG)

Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.

Bartosz Kula (B)

Del Monte Institute for Neuroscience, Department of Neuroscience, University of Rochester, School of Medicine and Dentistry, Rochester 14642, USA.

Nathan Smith (N)

Del Monte Institute for Neuroscience, Department of Neuroscience, University of Rochester, School of Medicine and Dentistry, Rochester 14642, USA.

Sharonda LeBlanc (S)

Department of Physics, North Carolina State University, Raleigh 27695, NC, USA; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill 27599, NC, USA.

Antentor Hinton (A)

Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA. Electronic address: antentor.o.hinton.jr@vanderbilt.edu.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › MitoTracker: A useful tool in need of better...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › MitoTracker: A useful tool in need of better...