Rapid and noninvasive cell assay by microfluidic-integrated intracavity evanescent field absorption in a fiber ring laser.

Microfluidics Biosensing Techniques / methods Fiber Optic Technology / methods Research Design Lasers
Cell assay Fiber-optic biosensor Intracavity absorption sensing

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
01 Dec 2023
Historique:
received: 16 08 2023
revised: 14 10 2023
accepted: 24 10 2023
medline: 27 11 2023
pubmed: 18 11 2023
entrez: 17 11 2023
Statut: ppublish

Résumé

Highly sensitive and rapid detection of cell concentration and interfacial molecular events is of great value for biological, biomedical, and chemical research. Most traditional biosensors require large sample volumes and complicated functional modifications of the surface. It is of great significance to develop label-free biosensor platforms with minimal sample consumption for studying cell concentration changes and interfacial molecular events without labor-intensive procedures. Here, a fiber-optic biosensor based on intracavity evanescent field absorption sensing is designed for sensitive and label-free cell assays for the first time. The interaction between the cells and the evanescent field is enhanced by introducing microfluidic-integrated intracavity absorption in a fiber ring laser. This strategy extends the range of targeted analytes to include quantification of a large number of targets on a surface and improves the detection sensitivity of the fiber-optic biosensor. The level of sensing resolution could be improved from 10 The proposed method suggests its capacity for true label-free and noninvasive cell assays with a low limit of detection and small sample consumption. This has the potential to be used as a universal tool for quantitative and qualitative characterization of various cells and other biochemical analytes.

Sections du résumé

BACKGROUND BACKGROUND
Highly sensitive and rapid detection of cell concentration and interfacial molecular events is of great value for biological, biomedical, and chemical research. Most traditional biosensors require large sample volumes and complicated functional modifications of the surface. It is of great significance to develop label-free biosensor platforms with minimal sample consumption for studying cell concentration changes and interfacial molecular events without labor-intensive procedures.
RESULTS RESULTS
Here, a fiber-optic biosensor based on intracavity evanescent field absorption sensing is designed for sensitive and label-free cell assays for the first time. The interaction between the cells and the evanescent field is enhanced by introducing microfluidic-integrated intracavity absorption in a fiber ring laser. This strategy extends the range of targeted analytes to include quantification of a large number of targets on a surface and improves the detection sensitivity of the fiber-optic biosensor. The level of sensing resolution could be improved from 10
SIGNIFICANCE CONCLUSIONS
The proposed method suggests its capacity for true label-free and noninvasive cell assays with a low limit of detection and small sample consumption. This has the potential to be used as a universal tool for quantitative and qualitative characterization of various cells and other biochemical analytes.

Identifiants

DOI: 10.1016/j.aca.2023.341960 PMID: 37977802
pubmed: 37977802
pii: S0003-2670(23)01181-9
doi: 10.1016/j.aca.2023.341960
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

341960

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors have no conflicts of interest to declare.

Auteurs

Jia Shi (J)

Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin, 300387, China; Key Laboratory of Opto-Electronics Information Technology (Ministry of Education), School of Precision Instruments and Opto-Electronic Engineering, Tianjin University, Tianjin, 300072, China. Electronic address: shijia@tiangong.edu.cn.

Ziyi Xu (Z)

Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin, 300387, China.

Ke Yang (K)

Department of Cardiovascular Surgery, General Hospital of Western Theater Command (Chengdu Military General Hospital), Chengdu, 610036, China. Electronic address: yangke@swjtu.edu.cn.

Xianguo Li (X)

Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin, 300387, China.

Cuijuan Guo (C)

Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin, 300387, China.

Hua Bai (H)

Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin, 300387, China.

Weiling Fu (W)

Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Pingjuan Niu (P)

Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin, 300387, China.

Jianquan Yao (J)

Key Laboratory of Opto-Electronics Information Technology (Ministry of Education), School of Precision Instruments and Opto-Electronic Engineering, Tianjin University, Tianjin, 300072, China.

Xiang Yang (X)

Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China. Electronic address: yangxiang@tmmu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Miniaturisation › Microtechnologie › Microfluidique › Rapid and noninvasive cell assay by...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Rapid and noninvasive cell assay by...
questionsmedicales.fr › Disciplines des sciences naturelles › Physique › Technologie des fibres optiques › Rapid and noninvasive cell assay by...
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Plan de recherche › Rapid and noninvasive cell assay by...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures pour les applications des radiations › Lasers › Rapid and noninvasive cell assay by...