Diadenosine tetraphosphate modulated quorum sensing in bacteria treated with kanamycin.
Biofilm
Diadenosine tetraphosphate
Kanamycin
Quorum sensing
apaH
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
17 Nov 2023
17 Nov 2023
Historique:
received:
14
09
2023
accepted:
06
11
2023
medline:
27
11
2023
pubmed:
18
11
2023
entrez:
18
11
2023
Statut:
epublish
Résumé
The dinucleotide alarmone diadenosine tetraphosphate (Ap4A), which is found in cells, has been shown to affect the survival of bacteria under stress. Here, we labeled Ap4A with biotin and incubated the labeled Ap4A with the total proteins extracted from kanamycin-treated Escherichia coli to identify the Ap4A binding protein in bacteria treated with kanamycin. Liquid chromatography‒mass spectrometry (LCMS) and bioinformatics were used to identify novel proteins that Ap4A interacts with that are involved in biofilm formation, quorum sensing, and lipopolysaccharide biosynthesis pathways. Then, we used the apaH knockout strain of E. coli K12-MG1655, which had increased intracellular Ap4A, to demonstrate that Ap4A affected the expression of genes in these three pathways. We also found that the swarming motility of the apaH mutant strain was reduced compared with that of the wild-type strain, and under kanamycin treatment, the biofilm formation of the mutant strain decreased. These results showed that Ap4A can reduce the survival rate of bacteria treated with kanamycin by regulating quorum sensing (QS). These effects can expand the application of kanamycin combinations in the treatment of multidrug-resistant bacteria.
Sections du résumé
BACKGROUND
BACKGROUND
The dinucleotide alarmone diadenosine tetraphosphate (Ap4A), which is found in cells, has been shown to affect the survival of bacteria under stress.
RESULTS
RESULTS
Here, we labeled Ap4A with biotin and incubated the labeled Ap4A with the total proteins extracted from kanamycin-treated Escherichia coli to identify the Ap4A binding protein in bacteria treated with kanamycin. Liquid chromatography‒mass spectrometry (LCMS) and bioinformatics were used to identify novel proteins that Ap4A interacts with that are involved in biofilm formation, quorum sensing, and lipopolysaccharide biosynthesis pathways. Then, we used the apaH knockout strain of E. coli K12-MG1655, which had increased intracellular Ap4A, to demonstrate that Ap4A affected the expression of genes in these three pathways. We also found that the swarming motility of the apaH mutant strain was reduced compared with that of the wild-type strain, and under kanamycin treatment, the biofilm formation of the mutant strain decreased.
CONCLUSIONS
CONCLUSIONS
These results showed that Ap4A can reduce the survival rate of bacteria treated with kanamycin by regulating quorum sensing (QS). These effects can expand the application of kanamycin combinations in the treatment of multidrug-resistant bacteria.
Identifiants
pubmed: 37978430
doi: 10.1186/s12866-023-03113-3
pii: 10.1186/s12866-023-03113-3
pmc: PMC10657157
doi:
Substances chimiques
Kanamycin
59-01-8
diadenosine tetraphosphate
5542-28-9
7-(N-(3-aminopropyl)amino)heptan-2-one
122269-09-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
353Subventions
Organisme : Foundation of Guangdong Educational Committee for Youths
ID : 2019KQNCX150
Organisme : Applied Basic Research Programs of Science and Technology Commission Foundation of Guangdong Province
ID : 2022A1515012602
Organisme : Foundation of Guangdong Educational Committee for Characteristic Innovation
ID : 2023KTSCX146
Informations de copyright
© 2023. The Author(s).
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