Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 Nov 2023
18 Nov 2023
Historique:
received:
03
04
2023
accepted:
07
11
2023
medline:
27
11
2023
pubmed:
19
11
2023
entrez:
18
11
2023
Statut:
epublish
Résumé
Bacterial chromosomes are dynamically and spatially organised within cells. In slow-growing Escherichia coli, the chromosomal terminus is initially located at the new pole and must therefore migrate to midcell during replication to reproduce the same pattern in the daughter cells. Here, we use high-throughput time-lapse microscopy to quantify this transition, its timing and its relationship to chromosome segregation. We find that terminus centralisation is a rapid discrete event that occurs ~25 min after initial separation of duplicated origins and ~50 min before the onset of bulk nucleoid segregation but with substantial variation between cells. Despite this variation, its movement is tightly coincident with the completion of origin segregation, even in the absence of its linkage to the divisome, suggesting a coupling between these two events. Indeed, we find that terminus centralisation does not occur if origin segregation away from mid-cell is disrupted, which results in daughter cells having an inverted chromosome organisation. Overall, our study quantifies the choreography of origin-terminus positioning and identifies an unexplored connection between these loci, furthering our understanding of chromosome segregation in this bacterium.
Identifiants
pubmed: 37980336
doi: 10.1038/s41467-023-43351-7
pii: 10.1038/s41467-023-43351-7
pmc: PMC10657355
doi:
Substances chimiques
Escherichia coli Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7489Informations de copyright
© 2023. The Author(s).
Références
J Bacteriol. 2007 Dec;189(23):8693-703
pubmed: 17905987
Cell. 2005 Jun 17;121(6):899-911
pubmed: 15960977
Mol Microbiol. 2006 Oct;62(2):331-8
pubmed: 17020576
Genes Dev. 2005 Oct 1;19(19):2367-77
pubmed: 16204186
Mol Microbiol. 2002 Nov;46(4):985-96
pubmed: 12421305
PLoS Genet. 2014 Jul 10;10(7):e1004448
pubmed: 25010199
Nat Rev Microbiol. 2010 Aug;8(8):600-7
pubmed: 20634810
PLoS Genet. 2014 Aug 07;10(8):e1004504
pubmed: 25101671
Annu Rev Cell Dev Biol. 2015;31:171-99
pubmed: 26566111
Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12877-82
pubmed: 25071173
PLoS Genet. 2013 May;9(5):e1003492
pubmed: 23658532
Curr Biol. 1998 Oct 8;8(20):1102-9
pubmed: 9778525
Curr Biol. 2010 Jun 22;20(12):1099-103
pubmed: 20537537
Nat Commun. 2017 Nov 28;8(1):1817
pubmed: 29180656
Proc Natl Acad Sci U S A. 2021 Aug 17;118(33):
pubmed: 34385314
Cell. 2018 Feb 8;172(4):771-783.e18
pubmed: 29358050
J Bacteriol. 1994 Sep;176(17):5320-9
pubmed: 8071208
Biophys J. 2016 Jun 21;110(12):2597-2609
pubmed: 27332118
Genes Dev. 2008 Sep 1;22(17):2426-33
pubmed: 18765793
mBio. 2017 Jun 6;8(3):
pubmed: 28588128
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2765-70
pubmed: 21282646
Cell. 2008 Sep 19;134(6):945-55
pubmed: 18805088
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9257-62
pubmed: 15178755
mBio. 2014 Feb 11;5(1):e01001-13
pubmed: 24520061
Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2214796120
pubmed: 36897981
Elife. 2021 Oct 06;10:
pubmed: 34612203
BMC Mol Cell Biol. 2020 May 1;21(1):35
pubmed: 32357828
Elife. 2022 Nov 14;11:
pubmed: 36374535
J Bacteriol. 2012 Sep;194(17):4669-76
pubmed: 22753058
Genes Dev. 2014 Jan 1;28(1):71-84
pubmed: 24395248
Cell. 2008 Sep 19;134(6):956-68
pubmed: 18805089
Front Microbiol. 2015 May 12;6:448
pubmed: 26029188
Genes Dev. 2000 Jan 15;14(2):212-23
pubmed: 10652275
Mol Microbiol. 2008 Jun;68(6):1418-27
pubmed: 18410497
Front Microbiol. 2021 Oct 26;12:721899
pubmed: 34795646
Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11157-62
pubmed: 23781109
Nucleic Acids Res. 2016 Feb 18;44(3):1216-26
pubmed: 26762981
Mol Microbiol. 2007 Sep;65(6):1485-92
pubmed: 17824928
Mol Microbiol. 2003 Aug;49(3):731-43
pubmed: 12864855
Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4991-5
pubmed: 20194778
Mol Microbiol. 2005 Jan;55(1):175-83
pubmed: 15612926
Cell. 2008 Oct 31;135(3):475-85
pubmed: 18984159
Nature. 1968 Sep 7;219(5158):1077-9
pubmed: 4876941
Mol Microbiol. 2006 Jul;61(2):383-93
pubmed: 16771843
Nat Commun. 2020 Jul 30;11(1):3796
pubmed: 32732900
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12388-93
pubmed: 16885211
Genes Dev. 2006 Jul 1;20(13):1727-31
pubmed: 16818605
Mol Cell. 2012 Nov 30;48(4):560-71
pubmed: 23084832
J Mol Biol. 2019 May 17;431(11):2061-2067
pubmed: 31026450
Mol Microbiol. 2012 Dec;86(6):1318-33
pubmed: 23078205
Genes Dev. 2006 Dec 1;20(23):3269-82
pubmed: 17158745
Open Biol. 2020 Jun;10(6):200097
pubmed: 32543349
Mol Microbiol. 2000 Nov;38(3):493-505
pubmed: 11069673
EMBO J. 2012 May 11;31(14):3198-211
pubmed: 22580828
Nat Commun. 2016 Jan 28;7:10466
pubmed: 26818444
Cell. 2013 May 9;153(4):882-95
pubmed: 23623305
Biophys J. 2023 May 2;122(9):1577-1585
pubmed: 36966362
Mol Microbiol. 2000 Aug;37(3):455-66
pubmed: 10931339
Front Microbiol. 2015 Apr 14;6:306
pubmed: 25926826
Genes Dev. 1998 Apr 1;12(7):1036-45
pubmed: 9531540
Elife. 2019 Aug 09;8:
pubmed: 31397672
Cell Rep. 2022 Mar 22;38(12):110539
pubmed: 35320717