Fluorescent molecular rotors as versatile in situ sensors for protein quantitation.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 Nov 2023
22 Nov 2023
Historique:
received:
05
07
2023
accepted:
02
11
2023
medline:
24
11
2023
pubmed:
23
11
2023
entrez:
22
11
2023
Statut:
epublish
Résumé
Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.
Identifiants
pubmed: 37993476
doi: 10.1038/s41598-023-46571-5
pii: 10.1038/s41598-023-46571-5
pmc: PMC10665405
doi:
Substances chimiques
Fluorescent Dyes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20529Subventions
Organisme : NIA NIH HHS
ID : RF1 AG062362
Pays : United States
Informations de copyright
© 2023. The Author(s).
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