Bioactive glasses promote rapid pre-osteoblastic cell migration in contrast to hydroxyapatite, while carbonated apatite shows migration inhibiting properties.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 Nov 2023
23 Nov 2023
Historique:
received:
02
06
2023
accepted:
20
11
2023
medline:
27
11
2023
pubmed:
24
11
2023
entrez:
23
11
2023
Statut:
epublish
Résumé
Different biomaterials have been clinically used as bone filling materials, although the mechanisms behind the biological effects are incompletely understood. To address this, we compared the effects of five different biomaterials: two bioactive glasses (45S5 and S53P4), hydroxyapatite (HAP), carbonated apatite (CAP), and alumina on the in vitro migration and viability of pre-osteoblastic cells. In addition, we studied the effects of biomaterials' calcium release on cell migration, viability and differentiation. We found differences between the materials as the bioactive glasses promoted rapid pre-osteoblastic cell migration. In contrast, CAP decreased cell migration, which was also associated with lower activity of migration related kinases. Bioactive glasses released significant amounts of calcium into the media, while CAP decreased the calcium concentration. The response of cells to calcium was mechanistically studied by blocking calcium sensing receptor (CaSR) and ATP-gated ion channel P2X7, but this had no effect on cell migration. Surprisingly, HAP and CAP initially decreased cell viability. In summary, bioactive glasses 45S5 and S53P4 had significant and long-lasting effects on the pre-osteoblastic cell migration, which could be related to the observed calcium dissolution. Additionally, bioactive glasses had no negative effects on cell viability, which was observed with HAP and CAP.
Identifiants
pubmed: 37996563
doi: 10.1038/s41598-023-47883-2
pii: 10.1038/s41598-023-47883-2
pmc: PMC10667509
doi:
Substances chimiques
Durapatite
91D9GV0Z28
Calcium
SY7Q814VUP
Biocompatible Materials
0
carboapatite
55326-60-8
Apatites
0
Calcium, Dietary
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20587Subventions
Organisme : Academy of Finland
ID : 323596
Organisme : Academy of Finland
ID : 323597
Organisme : Business Finland
ID : 11/31/2023
Informations de copyright
© 2023. The Author(s).
Références
Exp Mol Med. 2018 Nov 5;50(11):1-16
pubmed: 30393382
J Mol Endocrinol. 2015 Apr;54(2):R75-88
pubmed: 25591582
Cold Spring Harb Perspect Biol. 2015 Aug 03;7(8):a005959
pubmed: 26238352
J Tissue Eng Regen Med. 2020 Aug;14(8):1157-1168
pubmed: 32415757
Cell Biochem Biophys. 2013 Apr;65(3):455-62
pubmed: 23086356
Expert Rev Mol Med. 2011 Apr 26;13:e15
pubmed: 21545755
J Biomed Mater Res A. 2010 May;93(2):475-83
pubmed: 19582832
World Neurosurg. 2019 Apr;124:282-286
pubmed: 30684720
Biomaterials. 2011 Apr;32(11):2757-74
pubmed: 21292319
Dent Mater. 2022 Dec;38(12):1878-1885
pubmed: 36207169
J Appl Physiol (1985). 2007 Mar;102(3):1152-60
pubmed: 17122380
Int J Mol Sci. 2018 Aug 09;19(8):
pubmed: 30096908
Acta Neurochir (Wien). 2015 Apr;157(4):681-7
pubmed: 25663141
In Vitro Cell Dev Biol Anim. 2011 Mar;47(3):241-50
pubmed: 21136191
Acta Biomater. 2013 Sep;9(9):8037-45
pubmed: 23791671
PLoS One. 2019 Jan 4;14(1):e0210301
pubmed: 30608979
Acta Biomater. 2014 Jun;10(6):2824-33
pubmed: 24525034
J Mater Sci Mater Med. 2000 Jan;11(1):19-23
pubmed: 15348094
J Dent Res. 2012 Aug;91(8):736-44
pubmed: 22318372
Science. 2002 Feb 8;295(5557):1014-7
pubmed: 11834817
Biomaterials. 1991 Oct;12(8):752-6
pubmed: 1665992
Acta Biomater. 2013 Feb;9(2):5400-10
pubmed: 22967942
Exp Cell Res. 2010 Feb 1;316(3):443-51
pubmed: 19854170
Ann N Y Acad Sci. 1988;523:234-56
pubmed: 3382124
Dent Mater. 2021 Sep;37(9):1350-1357
pubmed: 34175132
Acta Biomater. 2008 Sep;4(5):1498-505
pubmed: 18502193
Birth Defects Res C Embryo Today. 2013 Sep;99(3):170-91
pubmed: 24078495
J Biomed Mater Res. 1998 Mar 5;39(3):390-7
pubmed: 9468047
J Mol Endocrinol. 2018 Jul;61(1):R1-R12
pubmed: 29599414
J Bone Miner Res. 1998 Oct;13(10):1530-8
pubmed: 9783541
Front Bioeng Biotechnol. 2015 Nov 30;3:194
pubmed: 26649290