Monitoring Phenotype Heterogeneity at the Single-Cell Level within


Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
05 Dec 2023
Historique:
medline: 6 12 2023
pubmed: 24 11 2023
entrez: 24 11 2023
Statut: ppublish

Résumé

Phenotypic heterogeneity is commonly found among bacterial cells within microbial populations due to intrinsic factors as well as equipping the organisms to respond to external perturbations. The emergence of phenotypic heterogeneity in bacterial populations, particularly in the context of using these bacteria as microbial cell factories, is a major concern for industrial bioprocessing applications. This is due to the potential impact on overall productivity by allowing the growth of subpopulations consisting of inefficient producer cells. Monitoring the spread of phenotypes across bacterial cells within the same population at the single-cell level is key to the development of robust, high-yield bioprocesses. Here, we discuss the novel development of optical photothermal infrared (O-PTIR) spectroscopy to probe phenotypic heterogeneity within

Identifiants

pubmed: 37997371
doi: 10.1021/acs.analchem.3c03595
pmc: PMC10701708
doi:

Substances chimiques

poly-beta-hydroxybutyrate 26063-00-3
Polyesters 0
Biopolymers 0
Hydroxybutyrates 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

17733-17740

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Auteurs

Cassio Lima (C)

Centre for Metabolomics Research, Department of Biochemistry, Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.

Howbeer Muhamadali (H)

Centre for Metabolomics Research, Department of Biochemistry, Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.

Royston Goodacre (R)

Centre for Metabolomics Research, Department of Biochemistry, Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.

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Classifications MeSH