Depletion of tRNA CCA-adding enzyme in Mycobacterium tuberculosis leads to polyadenylation of transcripts and precursor tRNAs.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 Nov 2023
Historique:
received: 11 10 2023
accepted: 20 11 2023
medline: 27 11 2023
pubmed: 25 11 2023
entrez: 24 11 2023
Statut: epublish

Résumé

In reference to gene annotation, more than half of the tRNA species synthesized by Mycobacterium tuberculosis require the enzymatic addition of the cytosine-cytosine-adenine (CCA) tail, which is indispensable for amino acid charging and tRNA functionality. It makes the mycobacterial CCA-adding enzyme essential for survival of the bacterium and a potential target for novel pipelines in drug discovery avenues. Here, we described the rv3907c gene product, originally annotated as poly(A)polymerase (rv3907c, PcnA) as a functional CCA-adding enzyme (CCA

Identifiants

pubmed: 38001315
doi: 10.1038/s41598-023-47944-6
pii: 10.1038/s41598-023-47944-6
pmc: PMC10673834
doi:

Substances chimiques

tRNA nucleotidyltransferase EC 2.7.7.-
Adenine JAC85A2161
Cytosine 8J337D1HZY
RNA Nucleotidyltransferases EC 2.7.7.-
RNA, Transfer 9014-25-9

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

20717

Subventions

Organisme : Narodowym Centrum Nauki
ID : UMO-2017/25/B/NZ7/01290
Organisme : Narodowym Centrum Nauki
ID : UMO-2019/34/E/NZ1/00338
Organisme : Narodowym Centrum Nauki
ID : UMO-2017/25/B/NZ7/01290
Organisme : Narodowym Centrum Nauki
ID : UMO-2017/25/B/NZ7/01290
Organisme : Narodowym Centrum Nauki
ID : UMO-2017/25/B/NZ7/01290

Informations de copyright

© 2023. The Author(s).

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Auteurs

Ewelina Błaszczyk (E)

Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland.

Przemysław Płociński (P)

Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland.
Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland.

Ewelina Lechowicz (E)

Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland.

Anna Brzostek (A)

Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland.

Bożena Dziadek (B)

Department of Molecular Microbiology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland.

Małgorzata Korycka-Machała (M)

Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland.

Marcin Słomka (M)

Biobank Lab, Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 139, 90-235, Łódź, Poland.

Jarosław Dziadek (J)

Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Łódź, Poland. jdziadek@cbm.pan.pl.

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Classifications MeSH