Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 Nov 2023
28 Nov 2023
Historique:
received:
30
07
2022
accepted:
30
10
2023
medline:
30
11
2023
pubmed:
29
11
2023
entrez:
28
11
2023
Statut:
epublish
Résumé
Plant-sourced aromatic amino acid (AAA) derivatives are a vast group of compounds with broad applications. Here, we present the development of a yeast consortium for efficient production of (S)-norcoclaurine, the key precursor for benzylisoquinoline alkaloid biosynthesis. A xylose transporter enables the concurrent mixed-sugar utilization in Scheffersomyces stipitis, which plays a crucial role in enhancing the flux entering the highly regulated shikimate pathway located upstream of AAA biosynthesis. Two quinate permeases isolated from Aspergillus niger facilitates shikimate translocation to the co-cultured Saccharomyces cerevisiae that converts shikimate to (S)-norcoclaurine, resulting in the maximal titer (11.5 mg/L), nearly 110-fold higher than the titer reported for an S. cerevisiae monoculture. Our findings magnify the potential of microbial consortium platforms for the economical de novo synthesis of complex compounds, where pathway modularization and compartmentalization in distinct specialty strains enable effective fine-tuning of long biosynthetic pathways and diminish intermediate buildup, thereby leading to increases in production.
Identifiants
pubmed: 38016984
doi: 10.1038/s41467-023-43049-w
pii: 10.1038/s41467-023-43049-w
pmc: PMC10684500
doi:
Substances chimiques
Xylose
A1TA934AKO
Benzylisoquinolines
0
shikimate
0
Membrane Transport Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7797Subventions
Organisme : National Science Foundation (NSF)
ID : 1716837
Organisme : National Science Foundation (NSF)
ID : EEC-0813570
Organisme : National Science Foundation (NSF)
ID : 1749782
Organisme : Iowa State University (Iowa State University of Science and Technology)
ID : PG110304
Informations de copyright
© 2023. The Author(s).
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