Dual-functionalization of fluorescent carbon dots via cyclodextrin and aminosilane for visual detection of β-glucuronidase and bioimaging.

Glucuronidase Cyclodextrins Quantum Dots Carbon Fluorescent Dyes
Carbon dots Fluorescence sensor Intracellular imaging Visual detection β-Glucuronidase

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
02 Jan 2024
Historique:
received: 01 08 2023
revised: 08 10 2023
accepted: 01 11 2023
medline: 11 12 2023
pubmed: 7 12 2023
entrez: 6 12 2023
Statut: ppublish

Résumé

A sensitive method for the detection of β-glucuronidase was established using functionalized carbon dots (β-CD-SiCDs) as fluorescent probes. The β-CD-SiCDs were found to be obtained through in situ autopolymerization by mixing the solutions of methyldopa, mono-6-ethylenediamine-β-cyclodextrin and N-(β-aminoethyl)-γ-aminopropyltrimethoxysilane at room temperature. The method has the characteristics of low energy consumption, simple and rapid. β-CD-SiCDs exhibited green fluorescence at 515 nm emission with a quantum yield of 7.9 %. 4-nitrophenyl-β-D-glucuronide was introduced as a substrate for β-glucuronidase to generate p-nitrophenol. Subsequently, p-nitrophenol self-assembled with β-CD-SiCDs through host-guest recognition to form a stable inclusion complex, resulting in the fluorescence quenching of β-CD-SiCDs. The linear range of β-CD-SiCDs for detecting β-glucuronidase activity was 0.5-60 U L

Identifiants

DOI: 10.1016/j.aca.2023.341996 PMID: 38057046
pubmed: 38057046
pii: S0003-2670(23)01217-5
doi: 10.1016/j.aca.2023.341996
pii:
doi:

Substances chimiques

4-nitrophenol Y92ZL45L4R
Glucuronidase EC 3.2.1.31
Cyclodextrins 0
Carbon 7440-44-0
Fluorescent Dyes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

341996

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Chenhui Yin (C)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Meng Wu (M)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Qijun Sun (Q)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Chenglin Su (C)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Shuang Cao (S)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Na Niu (N)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China. Electronic address: niuna@nefu.edu.cn.

Ligang Chen (L)

College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China. Electronic address: analchem@nefu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Glucuronidase › Dual-functionalization of fluorescent carbon...
questionsmedicales.fr › Glucides › Polyosides › Glucanes › Amidon › Dextrine › Cyclodextrines › Dual-functionalization of fluorescent carbon...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Boîtes quantiques › Dual-functionalization of fluorescent carbon...
questionsmedicales.fr › Produits chimiques inorganiques › Éléments › Carbone › Dual-functionalization of fluorescent carbon...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Dual-functionalization of fluorescent carbon...