Solid-phase peptide synthesis in 384-well plates.
384-well plate
SPPS
combinatorial chemistry
peptide library
peptide synthesis
Journal
Journal of peptide science : an official publication of the European Peptide Society
ISSN: 1099-1387
Titre abrégé: J Pept Sci
Pays: England
ID NLM: 9506309
Informations de publication
Date de publication:
Apr 2024
Apr 2024
Historique:
revised:
31
08
2023
received:
06
07
2023
accepted:
03
11
2023
medline:
18
3
2024
pubmed:
15
1
2024
entrez:
14
1
2024
Statut:
ppublish
Résumé
Newer solid-phase peptide synthesis and release strategies enable the production of short peptides with high purity, allowing direct screening for desired bioactivity without prior chromatographic purification. However, the maximum number of peptides that can currently be synthesized per microplate reactor is 96, allowing the parallel synthesis of 384 peptides in modern devices that have space for 4 microplate reactors. To synthesize larger numbers of peptides, we modified a commercially available peptide synthesizer to enable the production of peptides in 384-well plates, which allows the synthesis of 1,536 peptides in one run (4 × 384 peptides). We report new hardware components and customized software that allowed for the synthesis of 1,536 short peptides in good quantity (average > 0.5 μmol), at high concentration (average > 10 mM), and decent purity without purification (average > 80%). The high-throughput peptide synthesis, which we developed with peptide drug development in mind, may be widely used for peptide library synthesis and screening, antibody epitope scanning, epitope mimetic development, or protease/kinase substrate screening.
Substances chimiques
Peptide Library
0
Peptides
0
Epitopes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3555Subventions
Organisme : Swiss National Science Foundation
ID : 192368
Pays : Switzerland
Informations de copyright
© 2024 European Peptide Society and John Wiley & Sons, Ltd.
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