Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity.
BAM complex
E. coli
cell biology
outer membrane protein
protein assembly
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
16 Jan 2024
16 Jan 2024
Historique:
medline:
17
1
2024
pubmed:
16
1
2024
entrez:
16
1
2024
Statut:
epublish
Résumé
Outer membrane proteins (OMPs) are essential components of the outer membrane of Gram-negative bacteria. In terms of protein targeting and assembly, the current dogma holds that a 'β-signal' imprinted in the final β-strand of the OMP engages the β-barrel assembly machinery (BAM) complex to initiate membrane insertion and assembly of the OMP into the outer membrane. Here, we revealed an additional rule that signals equivalent to the β-signal are repeated in other, internal β-strands within bacterial OMPs, by peptidomimetic and mutational analysis. The internal signal is needed to promote the efficiency of the assembly reaction of these OMPs. BamD, an essential subunit of the BAM complex, recognizes the internal signal and the β-signal, arranging several β-strands and partial folding for rapid OMP assembly. The internal signal-BamD ordering system is not essential for bacterial viability but is necessary to retain the integrity of the outer membrane against antibiotics and other environmental insults.
Identifiants
pubmed: 38226797
doi: 10.7554/eLife.90274
pii: 90274
doi:
pii:
Substances chimiques
Bacterial Outer Membrane Proteins
0
Escherichia coli Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 21K15043
Organisme : Japan Agency for Medical Research and Development
ID : 23ama121029j0002
Organisme : Japan Society for the Promotion of Science
ID : 18K11543
Organisme : Japan Society for the Promotion of Science
ID : 21H03551
Organisme : National Health and Medical Research Council
ID : CDF1106798
Organisme : Australian Research Council
ID : DP160100227
Organisme : Japan Science and Technology Agency
ID : 10.52926/jpmjfr2064
Organisme : Japan Society for the Promotion of Science
ID : 19K16077
Organisme : Japan Society for the Promotion of Science
ID : 18KK0197
Organisme : Japan Society for the Promotion of Science
ID : 18H06052
Organisme : Japan Society for the Promotion of Science
ID : 22K12672
Organisme : Japan Society for the Promotion of Science
ID : 21KK0126
Organisme : National Health and Medical Research Council
ID : 2016330
Informations de copyright
© 2023, Germany et al.
Déclaration de conflit d'intérêts
EG, NT, KI, YM, RB, CS, RD, YD, YN, XL, CW, KH, KT, HS, TL, TS No competing interests declared
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