Enhanced Th17 responses in the appendix of children with complex compared to simple appendicitis are associated with microbial dysbiosis.
T cells
Th17
appendicitis
children
microbiota
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2023
2023
Historique:
received:
13
07
2023
accepted:
17
11
2023
medline:
22
1
2024
pubmed:
19
1
2024
entrez:
19
1
2024
Statut:
epublish
Résumé
Appendicitis is one of the most common causes of acute abdominal surgery in children. The clinical course of appendicitis ranges from simple to complex appendicitis. The mechanisms underlying the heterogeneity of appendicitis in children remain largely unclear. Dysregulated T cell responses play an important role in several inflammatory diseases of the intestine, but the extend of T cell dysregulation in appendicitis in children is less well known. To characterize appendiceal T cells in simple and complex appendicitis we performed in-depth immunophenotyping of appendiceal-derived T cells by flow cytometry and correlated this to appendiceal-derived microbiota analyses of the same patient. Appendix samples of twenty children with appendicitis (n = 8 simple, n = 12 complex) were collected. T cells in complex appendicitis displayed an increased differentiated phenotype compared to simple appendicitis, including a loss of both CD27 and CD28 by CD4 In conclusion, disruption of local T cell responses, and enhanced pro-inflammatory Th17 responses correlating to changes in the appendiceal microbiota were observed in children with complex compared to simple appendicitis. Further studies are needed to decipher the role of a dysregulated network of microbiota and Th17 cells in the development of complex appendicitis in children.
Identifiants
pubmed: 38239362
doi: 10.3389/fimmu.2023.1258363
pmc: PMC10794624
doi:
Substances chimiques
Interleukin-17
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1258363Informations de copyright
Copyright © 2024 The, Schreurs, Drewniak, Bakx, de Meij, Budding, Poort, Cense, Heij, van Heurn, Gorter and Bunders.
Déclaration de conflit d'intérêts
Author AB is shareholder of inBiome Amsterdam, The Netherlands. Author LP was employed by inBiome Amsterdam, The Netherlands. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Trends Mol Med. 2009 May;15(5):199-207
pubmed: 19362058
Med Sci (Basel). 2018 Dec 14;6(4):
pubmed: 30558253
Ann Emerg Med. 2000 Jul;36(1):39-51
pubmed: 10874234
J Theor Biol. 2007 Dec 21;249(4):826-31
pubmed: 17936308
Am J Surg. 2017 Dec;214(6):1195-1200
pubmed: 28941724
Cytometry A. 2008 Nov;73(11):975-83
pubmed: 18785267
J Crohns Colitis. 2019 Jul 25;13(7):905-915
pubmed: 30715262
J Immunol. 2012 Jun 15;188(12):6287-99
pubmed: 22566565
Eur J Clin Microbiol Infect Dis. 2014 Nov;33(11):1927-36
pubmed: 24894339
Immunity. 2009 Sep 18;31(3):389-400
pubmed: 19766082
J Immunol. 2019 Aug 15;203(4):946-955
pubmed: 31243092
Front Immunol. 2020 Jul 28;11:1558
pubmed: 32849528
J Immunol Methods. 2017 Jun;445:45-52
pubmed: 28274838
Sci Rep. 2020 Oct 15;10(1):17352
pubmed: 33060696
EMBO Mol Med. 2012 Feb;4(2):71-4
pubmed: 22278905
Front Immunol. 2020 Jul 14;11:1485
pubmed: 32760404
Nat Rev Microbiol. 2016 Jan;14(1):20-32
pubmed: 26499895
Nature. 1999 Oct 14;401(6754):708-12
pubmed: 10537110
Clin Exp Immunol. 2016 Oct;186(1):1-9
pubmed: 27271818
Clin Dev Immunol. 2007;2007:89017
pubmed: 18317533
Pediatr Infect Dis J. 2019 Oct;38(10):1054-1060
pubmed: 31568143
J Immunol. 2002 Jun 1;168(11):5954-8
pubmed: 12023402
FASEB J. 2010 Nov;24(11):4556-64
pubmed: 20643909
Eur J Immunol. 2017 Jun;47(6):946-953
pubmed: 28475283
Ann Surg. 2015 Jan;261(1):67-71
pubmed: 25072441
Nat Commun. 2020 Apr 22;11(1):1922
pubmed: 32321922
Front Immunol. 2018 Mar 01;9:339
pubmed: 29545794
Sci Rep. 2019 Sep 17;9(1):13473
pubmed: 31530835
Front Immunol. 2018 Oct 16;9:2394
pubmed: 30386342
Surgery. 2010 Mar;147(3):366-72
pubmed: 19892382
Exp Mol Med. 2017 May 26;49(5):e340
pubmed: 28546563
Eur J Pediatr. 2022 Dec;181(12):4221-4226
pubmed: 36195698
J Allergy Clin Immunol. 2015 Jan;135(1):217-27
pubmed: 25468195
Clin Exp Immunol. 2007 Oct;150(1):169-78
pubmed: 17680826
Annu Rev Immunol. 2019 Apr 26;37:521-546
pubmed: 30726153
Lancet. 2015 Sep 26;386(10000):1278-1287
pubmed: 26460662
Dev Comp Immunol. 2008;32(8):980-91
pubmed: 18329710
Am J Respir Crit Care Med. 2000 Oct;162(4 Pt 2):S185-9
pubmed: 11029392
Immunology. 2003 Aug;109(4):580-7
pubmed: 12871226
Immunity. 2019 Feb 19;50(2):462-476.e8
pubmed: 30770246
J Surg Res. 2017 Jun 15;214:190-196
pubmed: 28624043
J Pediatr Surg. 2017 Aug;52(8):1219-1227
pubmed: 28449821
Nat Immunol. 2007 Sep;8(9):942-9
pubmed: 17676045
Eur J Immunol. 2010 Jul;40(7):1830-5
pubmed: 20583029
J Clin Immunol. 2004 Mar;24(2):185-96
pubmed: 15024186
Sci Transl Med. 2017 Feb 8;9(376):
pubmed: 28179509
Inflamm Bowel Dis. 2020 Apr 11;26(5):653-661
pubmed: 31970388
Ann Diagn Pathol. 2000 Feb;4(1):46-58
pubmed: 10684382