The complete genome sequences of a negative single-stranded RNA virus and a double-stranded RNA virus coinfecting the entomopathogenic fungus Beauveria bassiana Vuillemin.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
08 Feb 2024
08 Feb 2024
Historique:
received:
18
10
2023
accepted:
11
01
2024
medline:
9
2
2024
pubmed:
9
2
2024
entrez:
9
2
2024
Statut:
epublish
Résumé
Beauveria bassiana Vuillemin is an entomopathogenic fungus that has been developed as a biological insecticide. B. bassiana can be infected by single or multiple mycoviruses, most of which are double-stranded RNA (dsRNA) viruses, while infections with single-stranded RNA (ssRNA) viruses, especially negative single-stranded RNA (-ssRNA) viruses, have been observed less frequently. In the present study, we sequenced and analyzed the complete genomes of two new different mycoviruses coinfecting a single B. bassiana strain: a -ssRNA virus which we have named "Beauveria bassiana negative-strand RNA virus 1" (BbNSRV1), and a dsRNA virus, which we have named "Beauveria bassiana orthocurvulavirus 1" (BbOCuV1). The genome of BbNSRV1 consists of a single segment of negative-sense, single-stranded RNA with a length of 6169 nt, containing a single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) with 1949 aa (220.1 kDa). BLASTx analysis showed that the RdRp had the highest sequence similarity (59.79%) to that of Plasmopara viticola lesion associated mononegaambi virus 2, a member of the family Mymonaviridae. This is the first report of a -ssRNA mycovirus infecting B. bassiana. The genome of BbOCuV1 consists of two dsRNA segments, 2164 bp and 1765 bp in length, respectively, with dsRNA1 encoding a protein with conserved RdRp motifs and 70.75% sequence identity to the putative RdRp of the taxonomically unassigned mycovirus Fusarium graminearum virus 5 (FgV5), and the dsRNA2 encoding a putative coat protein with sequence identity 64.26% to the corresponding protein of the FgV5. Phylogenetic analysis indicated that BbOCuV1 belongs to a taxonomically unassigned group of dsRNA mycoviruses related to members of the families Curvulaviridae and Partitiviridae. Hence, it might be the member of a new family that remains to be named and formally recognized.
Identifiants
pubmed: 38332318
doi: 10.1007/s00705-024-05985-w
pii: 10.1007/s00705-024-05985-w
doi:
Substances chimiques
RNA, Double-Stranded
0
RNA-Dependent RNA Polymerase
EC 2.7.7.48
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
42Subventions
Organisme : Jilin Project for Outstanding Talents (Teams) for Innovation and Entrepreneurshipin in Science and Technology
ID : 20230508011RC
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Kotta-Loizou I, Coutts RHA (2017) Studies on the virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence. PLOS Pathog 13:e1006183. https://doi.org/10.1371/journal.ppat.1006183
doi: 10.1371/journal.ppat.1006183
pubmed: 28114361
pmcid: 5293280
Li L, Kang Q, Zhang SB et al (2021) The whole genome sequence of a novel chrysovirus from Beauveria Bassiana Vuillemin, an entomopathogenic fungus. Arch Virol 3443–3447. https://doi.org/10.21203/rs.3.rs-488047/v1
Kang Q, Li L, Li J et al (2021) A novel polymycovirus with defective RNA isolated from the entomopathogenic fungus Beauveria bassiana Vuillemin. Arch Virol 166:3487–3492. https://doi.org/10.1007/s00705-021-05238-0
doi: 10.1007/s00705-021-05238-0
pubmed: 34623502
Filippou C, Garrido-Jurado I, Meyling NV et al (2018) Mycoviral population dynamics in Spanish isolates of the entomopathogenic fungus Beauveria bassiana. Viruses 10:665. https://doi.org/10.3390/v10120665
doi: 10.3390/v10120665
pubmed: 30477213
pmcid: 6315922
Shi N, Yang G, Wang P et al (2019) Complete genome sequence of a novel partitivirus from the entomogenous fungus Beauveria bassiana in China. Arch Virol 164:3141–3144. https://doi.org/10.1007/s00705-019-04428-1
doi: 10.1007/s00705-019-04428-1
pubmed: 31598844
Shi N, Hu F, Wang P et al (2021) Molecular characterization of two dsRNAs that could correspond to the genome of a new mycovirus that infects the entomopathogenic fungus Beauveria bassiana. Arch Virol 166:3233–3237. https://doi.org/10.1007/s00705-021-05239-z
doi: 10.1007/s00705-021-05239-z
pubmed: 34535823
Koloniuk I, Hrabáková L, Petrzik K (2015) Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:1585–1588. https://doi.org/10.1007/s00705-015-2416-0
doi: 10.1007/s00705-015-2416-0
pubmed: 25854690
Kondo H, Chiba S, Toyoda K, Suzuki N et al (2013) Evidence for negative-strand RNA virus infection in fungi. Virology 435:201–209. https://doi.org/10.1016/j.virol.2012.10.002
doi: 10.1016/j.virol.2012.10.002
pubmed: 23099204
Liu L, Xie J, Cheng J et al (2014) Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses. Proc Natl Acad Sci U S A 111:12205–12210. https://doi.org/10.1073/pnas.1401786111
doi: 10.1073/pnas.1401786111
pubmed: 25092337
pmcid: 4143027
Jiang et al (2022) ICTV Virus Taxonomy Profile: Mymonaviridae 2022. JGV 103. https://doi.org/10.1099/jgv.0.001787
Chiapello M, Rodríguez-Romero J, Ayllón MA et al (2020) Analysis of the virome associated to grapevine downy mildew lesions reveals new mycovirus lineages. Virus Evol 6:veaa058. https://doi.org/10.1093/ve/veaa058
doi: 10.1093/ve/veaa058
pubmed: 33324489
pmcid: 7724247
Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858. https://doi.org/10.1094/Phyto-69-854
doi: 10.1094/Phyto-69-854
Pankovics P, Boros Á, Reuter G (2015) Novel 5’/3'RACE method for amplification and determination of single-stranded RNAs through double-stranded RNA (dsRNA) intermediates. Mol Biotechnol 57:974–981. https://doi.org/10.1007/s12033-015-9889-7
doi: 10.1007/s12033-015-9889-7
pubmed: 26315976
Darissa O, Willingmann P, Adam G (2010) Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods 169:397–403. https://doi.org/10.1016/j.jviromet.2010.08.013
doi: 10.1016/j.jviromet.2010.08.013
pubmed: 20727370
Cho WK, Lee KM, Yu J et al (2013) Insight into mycoviruses infecting Fusarium species. Adv Virus Res 86:273–288. https://doi.org/10.1016/b978-0-12-394315-6.00010-6
doi: 10.1016/b978-0-12-394315-6.00010-6
pubmed: 23498910
Wang L et al (2017) The complete genome sequence of a double-stranded RNA mycovirus from Fusarium Graminearum Strain Hn1. Arch Virol 7:2119–2124. https://doi.org/10.1007/s00705-017-3317-1
doi: 10.1007/s00705-017-3317-1