Dynamics of DNA damage-induced nuclear inclusions are regulated by SUMOylation of Btn2.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 Apr 2024
13 Apr 2024
Historique:
received:
16
08
2023
accepted:
05
04
2024
medline:
15
4
2024
pubmed:
14
4
2024
entrez:
13
4
2024
Statut:
epublish
Résumé
Spatial compartmentalization is a key facet of protein quality control that serves to store disassembled or non-native proteins until triage to the refolding or degradation machinery can occur in a regulated manner. Yeast cells sequester nuclear proteins at intranuclear quality control bodies (INQ) in response to various stresses, although the regulation of this process remains poorly understood. Here we reveal the SUMO modification of the small heat shock protein Btn2 under DNA damage and place Btn2 SUMOylation in a pathway promoting protein clearance from INQ structures. Along with other chaperones, and degradation machinery, Btn2-SUMO promotes INQ clearance from cells recovering from genotoxic stress. These data link small heat shock protein post-translational modification to the regulation of protein sequestration in the yeast nucleus.
Identifiants
pubmed: 38615096
doi: 10.1038/s41467-024-47615-8
pii: 10.1038/s41467-024-47615-8
pmc: PMC11016081
doi:
Substances chimiques
BTN2 protein, S cerevisiae
0
Heat-Shock Proteins, Small
0
Saccharomyces cerevisiae Proteins
0
Vesicular Transport Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3215Subventions
Organisme : Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada)
ID : RPGIN 2020-04360
Organisme : Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada)
ID : MOP136982
Informations de copyright
© 2024. The Author(s).
Références
J Biol Chem. 2022 Aug;298(8):102199
pubmed: 35760103
Cell Rep. 2019 Jan 2;26(1):236-249.e4
pubmed: 30605679
J Biol Chem. 2007 Nov 23;282(47):34167-75
pubmed: 17728242
Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2213703120
pubmed: 36574706
Hum Mol Genet. 2005 May 15;14(10):1351-65
pubmed: 15829507
Mol Cell. 2012 Feb 10;45(3):422-32
pubmed: 22285753
Science. 2016 Jan 8;351(6269):173-6
pubmed: 26634439
Elife. 2020 Sep 17;9:
pubmed: 32940602
Mol Biol Cell. 2012 Aug;23(16):3041-56
pubmed: 22718905
Nat Cell Biol. 2012 Sep;14(9):966-76
pubmed: 22842922
Front Neurosci. 2017 Apr 06;11:185
pubmed: 28428740
Cell. 2005 Nov 18;123(4):581-92
pubmed: 16286007
Front Mol Biosci. 2021 May 07;8:673038
pubmed: 34026847
Nat Commun. 2019 Dec 9;10(1):5611
pubmed: 31819057
EMBO J. 2001 Jul 2;20(13):3544-53
pubmed: 11432841
Mol Cell. 2014 Jul 3;55(1):15-30
pubmed: 24882209
Front Mol Biosci. 2023 Jan 04;9:1106477
pubmed: 36660429
Curr Med Chem. 2008;15(1):47-60
pubmed: 18220762
Nat Commun. 2019 Oct 24;10(1):4851
pubmed: 31649258
J Mol Biol. 2023 Jul 1;435(13):168139
pubmed: 37146746
J Biol Chem. 2004 Jan 2;279(1):223-30
pubmed: 14573610
Mol Cell Biol. 2007 Apr;27(8):3199-210
pubmed: 17296735
Bioinformatics. 2015 Nov 1;31(21):3483-91
pubmed: 26142185
Cell Rep. 2020 Jun 2;31(9):107680
pubmed: 32492414
Elife. 2021 Jan 27;10:
pubmed: 33502312
Genome Biol. 2009;10(5):R57
pubmed: 19470158
J Cell Sci. 2020 Dec 3;133(23):
pubmed: 33172985
J Biol Chem. 1985 Nov 25;260(27):14873-8
pubmed: 2997227
EMBO J. 2017 Mar 15;36(6):783-796
pubmed: 28219929
J Cell Biol. 2011 Nov 14;195(4):617-29
pubmed: 22065637
Nucleic Acids Res. 2019 Apr 23;47(7):3521-3535
pubmed: 30668788
Nucleic Acids Res. 2015 Jul 1;43(W1):W306-13
pubmed: 25883144
Nature. 2008 Aug 28;454(7208):1088-95
pubmed: 18756251
Mol Cell. 2013 Jun 6;50(5):625-36
pubmed: 23644018
Annu Rev Biochem. 2018 Jun 20;87:725-749
pubmed: 29925261
J Cell Biol. 2022 Oct 3;221(10):
pubmed: 36069810
Cell Rep. 2017 Nov 21;21(8):2147-2159
pubmed: 29166606
Nat Commun. 2018 Nov 27;9(1):5016
pubmed: 30479332
J Mol Biol. 2010 Sep 10;402(1):70-82
pubmed: 20643142
Mol Biol Cell. 2001 Oct;12(10):2987-3003
pubmed: 11598186
EMBO J. 2012 Feb 15;31(4):883-94
pubmed: 22234185
Curr Biol. 2012 Jan 10;22(1):56-63
pubmed: 22177115
Trends Biochem Sci. 2015 Apr;40(4):233-42
pubmed: 25778614
Mol Cell. 2020 Jul 2;79(1):54-67.e7
pubmed: 32521226
Cell Rep. 2016 May 10;15(6):1254-65
pubmed: 27134171
Protein Expr Purif. 2005 Sep;43(1):1-9
pubmed: 16084395
PLoS Genet. 2013;9(8):e1003670
pubmed: 23935535
STAR Protoc. 2022 Jul 19;3(3):101571
pubmed: 35880124
J Cell Biol. 2018 Apr 2;217(4):1269-1285
pubmed: 29362223
Nat Commun. 2015 Mar 30;6:6533
pubmed: 25817432
Cell Rep. 2017 Aug 15;20(7):1553-1562
pubmed: 28813668
EMBO J. 2008 Jan 23;27(2):336-49
pubmed: 18216876
PLoS Genet. 2015 Jan 08;11(1):e1004899
pubmed: 25569253
Annu Rev Biochem. 2017 Jun 20;86:97-122
pubmed: 28489421
Mol Cell Biol. 2013 Oct;33(19):3735-48
pubmed: 23878396
Cell. 2005 Nov 18;123(4):593-605
pubmed: 16286008
Science. 2002 Nov 15;298(5597):1412-4
pubmed: 12434058
Cell. 2012 Nov 9;151(4):807-820
pubmed: 23122649
J Cell Biol. 2017 Dec 4;216(12):4027-4040
pubmed: 28978642
Genes Dev. 2016 Apr 15;30(8):931-45
pubmed: 27056668
Nat Cell Biol. 2013 Oct;15(10):1231-43
pubmed: 24036477
Environ Mol Mutagen. 2017 Jun;58(5):235-263
pubmed: 28485537
Mol Cell. 2019 Nov 21;76(4):632-645.e6
pubmed: 31519521
Nat Neurosci. 2018 Feb;21(2):228-239
pubmed: 29311743
Nucleic Acids Res. 2019 Jan 8;47(D1):D1245-D1249
pubmed: 30357397
J Biol Chem. 2008 Jul 18;283(29):19912-21
pubmed: 18499666
EMBO J. 2018 Nov 2;37(21):
pubmed: 30287420
Mol Cell Biol. 2004 Jun;24(11):4769-80
pubmed: 15143171
Nat Cell Biol. 2023 May;25(5):699-713
pubmed: 37081164
EMBO J. 2015 Mar 12;34(6):778-97
pubmed: 25672362
PLoS Genet. 2011 Apr;7(4):e1002057
pubmed: 21552543