Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization.
Phytochrome B
/ metabolism
Arabidopsis Proteins
/ metabolism
Arabidopsis
/ metabolism
Basic Helix-Loop-Helix Transcription Factors
/ metabolism
Signal Transduction
Proteolysis
/ radiation effects
Light
Protein Stability
Gene Expression Regulation, Plant
Cell Nucleus
/ metabolism
Plants, Genetically Modified
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 Apr 2024
25 Apr 2024
Historique:
received:
29
09
2023
accepted:
10
04
2024
medline:
26
4
2024
pubmed:
26
4
2024
entrez:
25
4
2024
Statut:
epublish
Résumé
Photoactivation of the plant photoreceptor and thermosensor phytochrome B (PHYB) triggers its condensation into subnuclear membraneless organelles named photobodies (PBs). However, the function of PBs in PHYB signaling remains frustratingly elusive. Here, we found that PHYB recruits PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) to PBs. Surprisingly, PHYB exerts opposing roles in degrading and stabilizing PIF5. Perturbing PB size by overproducing PHYB provoked a biphasic PIF5 response: while a moderate increase in PHYB enhanced PIF5 degradation, further elevating the PHYB level stabilized PIF5 by retaining more of it in enlarged PBs. Conversely, reducing PB size by dim light, which enhanced PB dynamics and nucleoplasmic PHYB and PIF5, switched the balance towards PIF5 degradation. Together, these results reveal that PB formation spatially segregates two antagonistic PHYB signaling actions - PIF5 stabilization in PBs and PIF5 degradation in the surrounding nucleoplasm - which could enable an environmentally sensitive, counterbalancing mechanism to titrate nucleoplasmic PIF5 and environmental responses.
Identifiants
pubmed: 38664420
doi: 10.1038/s41467-024-47790-8
pii: 10.1038/s41467-024-47790-8
doi:
Substances chimiques
Phytochrome B
136250-22-1
Arabidopsis Proteins
0
Basic Helix-Loop-Helix Transcription Factors
0
PHYB protein, Arabidopsis
0
PIF5 protein, Arabidopsis
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3519Subventions
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)
ID : R01GM087388
Informations de copyright
© 2024. The Author(s).
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