Human nasal microbiota shifts in healthy and chronic respiratory disease conditions.
Humans
Microbiota
Female
Male
RNA, Ribosomal, 16S
/ genetics
Middle Aged
Adult
Bacteria
/ classification
Chronic Disease
Bronchoalveolar Lavage Fluid
/ microbiology
Nasopharynx
/ microbiology
Respiratory Tract Diseases
/ microbiology
Lithuania
Nose
/ microbiology
Aged
Young Adult
Nasal Cavity
/ microbiology
Sequence Analysis, DNA
/ methods
Healthy Volunteers
Biomarkers
Nasal microbiota
Upper respiratory tract
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
27 Apr 2024
27 Apr 2024
Historique:
received:
30
11
2023
accepted:
04
04
2024
medline:
28
4
2024
pubmed:
28
4
2024
entrez:
27
4
2024
Statut:
epublish
Résumé
An increasing number of studies investigate various human microbiotas and their roles in the development of diseases, maintenance of health states, and balanced signaling towards the brain. Current data demonstrate that the nasal microbiota contains a unique and highly variable array of commensal bacteria and opportunistic pathogens. However, we need to understand how to harness current knowledge, enrich nasal microbiota with beneficial microorganisms, and prevent pathogenic developments. In this study, we have obtained nasal, nasopharyngeal, and bronchoalveolar lavage fluid samples from healthy volunteers and patients suffering from chronic respiratory tract diseases for full-length 16 S rRNA sequencing analysis using Oxford Nanopore Technologies. Demographic and clinical data were collected simultaneously. The microbiome analysis of 97 people from Lithuania suffering from chronic inflammatory respiratory tract disease and healthy volunteers revealed that the human nasal microbiome represents the microbiome of the upper airways well. The nasal microbiota of patients was enriched with opportunistic pathogens, which could be used as indicators of respiratory tract conditions. In addition, we observed that a healthy human nasal microbiome contained several plant- and bee-associated species, suggesting the possibility of enriching human nasal microbiota via such exposures when needed. These candidate probiotics should be investigated for their modulating effects on airway and lung epithelia, immunogenic properties, neurotransmitter content, and roles in maintaining respiratory health and nose-brain interrelationships.
Sections du résumé
BACKGROUND
BACKGROUND
An increasing number of studies investigate various human microbiotas and their roles in the development of diseases, maintenance of health states, and balanced signaling towards the brain. Current data demonstrate that the nasal microbiota contains a unique and highly variable array of commensal bacteria and opportunistic pathogens. However, we need to understand how to harness current knowledge, enrich nasal microbiota with beneficial microorganisms, and prevent pathogenic developments.
RESULTS
RESULTS
In this study, we have obtained nasal, nasopharyngeal, and bronchoalveolar lavage fluid samples from healthy volunteers and patients suffering from chronic respiratory tract diseases for full-length 16 S rRNA sequencing analysis using Oxford Nanopore Technologies. Demographic and clinical data were collected simultaneously. The microbiome analysis of 97 people from Lithuania suffering from chronic inflammatory respiratory tract disease and healthy volunteers revealed that the human nasal microbiome represents the microbiome of the upper airways well.
CONCLUSIONS
CONCLUSIONS
The nasal microbiota of patients was enriched with opportunistic pathogens, which could be used as indicators of respiratory tract conditions. In addition, we observed that a healthy human nasal microbiome contained several plant- and bee-associated species, suggesting the possibility of enriching human nasal microbiota via such exposures when needed. These candidate probiotics should be investigated for their modulating effects on airway and lung epithelia, immunogenic properties, neurotransmitter content, and roles in maintaining respiratory health and nose-brain interrelationships.
Identifiants
pubmed: 38678223
doi: 10.1186/s12866-024-03294-5
pii: 10.1186/s12866-024-03294-5
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
150Subventions
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Organisme : Lietuvos Mokslo Taryba
ID : 01.2.2-LMT-K-718-03-0079
Informations de copyright
© 2024. The Author(s).
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