Wastewater treatment bacteria show differential preference for colonizing natural biopolymers.
Activated sludge
Biofilm
Cellulose
Chitin
Keratin
Lignin
Lignocellulose
Nitrogen
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
06 May 2024
06 May 2024
Historique:
received:
02
03
2024
accepted:
25
04
2024
revised:
21
04
2024
medline:
6
5
2024
pubmed:
6
5
2024
entrez:
6
5
2024
Statut:
epublish
Résumé
Most reduced organic matter entering activated sludge systems is particulate (1-100-µm diameter) or colloidal (0.001-1-µm diameter), yet little is known about colonization of particulate organic matter by activated sludge bacteria. In this study, colonization of biopolymers (chitin, keratin, lignocellulose, lignin, and cellulose) by activated sludge bacteria was compared with colonization of glass beads in the presence and absence of regular nutrient amendment (acetate and ammonia). Scanning electron microscopy and quantitative PCR revealed chitin and cellulose were most readily colonized followed by lignin and lignocellulose, while keratin and glass beads were relatively resistant to colonization. Bacterial community profiles on particles compared to sludge confirmed that specific bacterial phylotypes preferentially colonize different biopolymers. Nitrifying bacteria proved adept at colonizing particles, achieving higher relative abundance on particles compared to bulk sludge. Denitrifying bacteria showed similar or lower relative abundance on particles compared to sludge. KEY POINTS: • Some activated sludge bacteria colonize natural biopolymers more readily than others. • Nitrifying bacteria are overrepresented in natural biopolymer biofilm communities. • Biopolymers in wastewater likely influence activated sludge community composition.
Identifiants
pubmed: 38709299
doi: 10.1007/s00253-024-13162-x
pii: 10.1007/s00253-024-13162-x
doi:
Substances chimiques
Biopolymers
0
Sewage
0
Wastewater
0
Lignin
9005-53-2
Cellulose
9004-34-6
Chitin
1398-61-4
lignocellulose
11132-73-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
321Subventions
Organisme : Australian Research Council
ID : LP180100953
Informations de copyright
© 2024. The Author(s).
Références
Ahmed AS, Bahreini G, Ho D, Sridhar G, Gupta M, Wessels C, Marcelis P, Elbeshbishy E, Rosso D, Santoro D (2019) Fate of cellulose in primary and secondary treatment at municipal water resource recovery facilities. Water Environ Res 91(11):1479–1489. https://doi.org/10.1002/wer.1145
doi: 10.1002/wer.1145
pubmed: 31099937
Baird RB, Bridgewater L (2017) Standard methods for the examination of water and wastewater, 23rd. Water Environment Federation, American Public Health Association
Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Lozupone CA, Turnbaugh PJ, Fierer N, Knight R (2011) Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. P Natl Acad Sci USA 108(Supplement 1):4516–4522. https://doi.org/10.1073/pnas.1000080107
doi: 10.1073/pnas.1000080107
Chukwuma OB, Rafatullah M, Tajarudin HA, Ismail N (2021) A review on bacterial contribution to lignocellulose breakdown into useful bio-products. Int J Env Res Pub He 18(11):6001. https://doi.org/10.3390/ijerph18116001
doi: 10.3390/ijerph18116001
Dang HT, Dinh CV, Nguyen KM, Tran NT, Pham TT, Narbaitz RM (2020) Loofah sponges as bio-carriers in a pilot-scale integrated fixed-film activated sludge system for municipal wastewater treatment. Sustainability 12(11):4758. https://doi.org/10.3390/su12114758
doi: 10.3390/su12114758
Dekker RF (1985) Chapter 18: Biodegradation of the hemicelluloses. In: Higuchi T (ed) Biosynthesis and biodegradation of wood components, 1st Edn. Academic Press. https://doi.org/10.1016/b978-0-12-347880-1.50022-2
Dottorini G, Michaelsen TY, Kucheryavskiy S, Andersen KS, Kristensen JM, Peces M, Wagner DS, Nierychlo M, Nielsen PH (2021) Mass-immigration determines the assembly of activated sludge microbial communities. P Natl Acad Sci USA 118(27):e2021589118. https://doi.org/10.1073/pnas.2021589118
doi: 10.1073/pnas.2021589118
Felföldi T, Jurecska L, Vajna B, Barkacs K, Makk J, Cebe G, Szabo A, Záray G, Márialigeti K (2015) Texture and type of polymer fiber carrier determine bacterial colonization and biofilm properties in wastewater treatment. Chem Eng J 264:824–834. https://doi.org/10.1016/j.cej.2014.12.008
doi: 10.1016/j.cej.2014.12.008
Flint HJ, Scott KP, Duncan SH, Louis P, Forano E (2012) Microbial degradation of complex carbohydrates in the gut. Gut Microbes 3(4):289–306. https://doi.org/10.4161/gmic.19897
doi: 10.4161/gmic.19897
pubmed: 22572875
pmcid: 3463488
Gooday GW (1994) Physiology of microbial degradation of chitin and chitosan. In: Ratledge C (ed) Biochemistry of microbial degradation. Springer, Dordrecht, pp 279–312. https://doi.org/10.1007/978-94-011-1687-9_9
Hazrin-Chong NH, Manefield M (2012) An alternative SEM drying method using hexamethyldisilazane (HMDS) for microbial cell attachment studies on sub-bituminous coal. J Microbiol Methods 90(2):96–99. https://doi.org/10.1016/j.mimet.2012.04.014
doi: 10.1016/j.mimet.2012.04.014
pubmed: 22561094
Horz H, Vianna M, Gomes B, Conrads G (2005) Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy. J Clin Microbiol 43(10):5332–5337. https://doi.org/10.1128/jcm.43.10.5332-5337.2005
doi: 10.1128/jcm.43.10.5332-5337.2005
pubmed: 16208011
pmcid: 1248440
Kaplan DL (1998) Biopolymers from renewable resources. Spinger, Berlin. https://doi.org/10.1007/978-3-662-03680-8
Kelly JJ, London MG, McCormick AR, Rojas M, Scott JW, Hoellein TJ (2021) Wastewater treatment alters microbial colonization of microplastics. PLoS ONE 16(1):e0244443. https://doi.org/10.1371/journal.pone.0244443
doi: 10.1371/journal.pone.0244443
pubmed: 33406095
pmcid: 7787475
Khatoon N, Naz I, Ali MI, Ali N, Jamal A, Hameed A, Ahmed S (2014) Bacterial succession and degradative changes by biofilm on plastic medium for wastewater treatment. J Basic Microbiol 54(7):739–749. https://doi.org/10.1002/jobm.201300162
doi: 10.1002/jobm.201300162
pubmed: 24115187
Lee S-H, Hong TI, Kim B, Hong S, Park H-D (2014) Comparison of bacterial communities of biofilms formed on different membrane surfaces. World J Microbiol Biotechnol 30(2):777–782. https://doi.org/10.1007/s11274-013-1460-8
doi: 10.1007/s11274-013-1460-8
pubmed: 23975695
Levine AD, Tchobanoglous G, Asano T (1985) Characterization of the size distribution of contaminants in wastewater: treatment and reuse implications. J Water Pollut Control Fed 57(7):805–816. https://www.jstor.org/stable/25042701
Maki M, Leung KT, Qin W (2009) The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci 5(5):500. https://doi.org/10.7150/ijbs.5.500
doi: 10.7150/ijbs.5.500
pubmed: 19680472
pmcid: 2726447
Maunders E, Welch M (2017) Matrix exopolysccharides; the sticky side of biofilm formation. FEMS Microbiol Lett 364(13). https://doi.org/10.1093/femsle/fnx120
McIlroy SJ, Starnawska A, Starnawski P, Saunders AM, Nierychlo M, Nielsen PH, Nielsen JL (2016) Identification of active denitrifiers in full-scale nutrient removal wastewater treatment systems. Environ Microbiol 18(1):50–64. https://doi.org/10.1111/1462-2920.12614
doi: 10.1111/1462-2920.12614
pubmed: 25181571
Metcalf & Eddy (2014) Wastewater engineering: treatment and resource recovery, 5th edn. McGraw-Hill, New York
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5(1):62–71. https://doi.org/10.1006/niox.2000.0319
doi: 10.1006/niox.2000.0319
pubmed: 11178938
Naz I, Batool SA-u, Ali N, Khatoon N, Atiq N, Hameed A, Ahmed S (2013) Monitoring of growth and physiological activities of biofilm during succession on polystyrene from activated sludge under aerobic and anaerobic conditions. Environ Monit Assess 185(8):6881–6892. https://doi.org/10.1007/s10661-013-3072-z
doi: 10.1007/s10661-013-3072-z
pubmed: 23361646
Niestępski S, Harnisz M, Ciesielski S, Korzeniewska E, Osińska A (2020) Environmental fate of Bacteroidetes, with particular emphasis on Bacteroides fragilis group bacteria and their specific antibiotic resistance genes, in activated sludge wastewater treatment plants. J Hazard Mater 394:122544. https://doi.org/10.1016/j.jhazmat.2020.122544
doi: 10.1016/j.jhazmat.2020.122544
pubmed: 32224375
Palanisamy V, Gajendiran V, Mani K (2022) Meta-analysis to identify the core microbiome in diverse wastewater. Int J Environ Sci Technol 19:5079–5096. https://doi.org/10.1007/s13762-021-03349-4
Ransom-Jones E, Jones DL, McCarthy AJ, McDonald JE (2012) The Fibrobacteres: an important phylum of cellulose-degrading bacteria. Microb Ecol 63(2):267–281. https://doi.org/10.1007/s00248-011-9998-1
doi: 10.1007/s00248-011-9998-1
pubmed: 22213055
Raut MP, Karunakaran E, Mukherjee J, Biggs CA, Wright PC (2015) Influence of substrates on the surface characteristics and membrane proteome of Fibrobacter succinogenes S85. PLOS ONE 10(10):e0141197. https://doi.org/10.1371/journal.pone.0141197
doi: 10.1371/journal.pone.0141197
pubmed: 26492413
pmcid: 4619616
Scarlett K, Denman S, Clark DR, Forster J, Vanguelova E, Brown N, Whitby C (2021) Relationships between nitrogen cycling microbial community abundance and composition reveal the indirect effect of soil pH on oak decline. ISME J 15(3):623–635. https://doi.org/10.1038/s41396-020-00801-0
doi: 10.1038/s41396-020-00801-0
pubmed: 33067585
Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C (2011) Metagenomic biomarker discovery and explanation. Genome Biol 12:1–18. https://doi.org/10.1186/gb-2011-12-6-r60
doi: 10.1186/gb-2011-12-6-r60
Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol 13(3):218–227. https://doi.org/10.1016/s0958-1669(02)00315-4
doi: 10.1016/s0958-1669(02)00315-4
pubmed: 12180096
Wang P, Zhang H, Zuo J, Zhao D, Zou X, Zhu Z, Jeelani N, Leng X, An S (2016) A hardy plant facilitates nitrogen removal via microbial communities in subsurface flow constructed wetlands in winter. Sci Rep 6(1):1–11. https://doi.org/10.1038/srep33600
doi: 10.1038/srep33600
Wu L, Ning D, Zhang B, Li Y, Zhang P, Shan X, Zhang Q, Brown MR, Li Z, Van Nostrand JD (2019) Global diversity and biogeography of bacterial communities in wastewater treatment plants. Nat Microbiol 4(7):1183–1195. https://doi.org/10.1038/s41564-019-0426-5
doi: 10.1038/s41564-019-0426-5
pubmed: 31086312