Diversity and Bioprospection of Gram-positive Bacteria Derived from a Mayan Sinkhole.
16S rRNA
Agar Plate Assays
Antimicrobial Activity
Gram-positive Bacteria
Hydrolytic Enzymes
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
29 May 2024
29 May 2024
Historique:
received:
03
03
2024
accepted:
17
05
2024
medline:
29
5
2024
pubmed:
29
5
2024
entrez:
28
5
2024
Statut:
epublish
Résumé
Water-filled sinkholes known locally as cenotes, found on the Yucatán Peninsula, have remarkable biodiversity. The primary objective of this study was to explore the biotechnological potential of Gram-positive cultivable bacteria obtained from sediment samples collected at the coastal cenote Pol-Ac in Yucatán, Mexico. Specifically, the investigation aimed to assess production of hydrolytic enzymes and antimicrobial compounds. 16 S rRNA gene sequencing led to the identification of 49 Gram-positive bacterial isolates belonging to the phyla Bacillota (n = 29) and Actinomycetota (n = 20) divided into the common genera Bacillus and Streptomyces, as well as the genera Virgibacillus, Halobacillus, Metabacillus, Solibacillus, Neobacillus, Rossellomorea, Nocardiopsis and Corynebacterium. With growth at 55ºC, 21 of the 49 strains were classified as moderately thermotolerant. All strains were classified as halotolerant and 24 were dependent on marine water for growth. Screening for six extracellular hydrolytic enzymes revealed gelatinase, amylase, lipase, cellulase, protease and chitinase activities in 93.9%, 67.3%, 63.3%, 59.2%, 59.2% and 38.8%, of isolated strains, respectively. The genes for polyketide synthases type I, were detected in 24 of the strains. Of 18 strains that achieved > 25% inhibition of growth in the bacterial pathogen Staphylococcus aureus ATCC 6538, 4 also inhibited growth in Escherichia coli ATCC 35,218. Isolates Streptomyces sp. NCA_378 and Bacillus sp. NCA_374 demonstrated 50-75% growth inhibition against at least one of the two pathogens tested, along with significant enzymatic activity across all six extracellular enzymes. This is the first comprehensive report on the biotechnological potential of Gram-positive bacteria isolated from sediments in the cenotes of the Yucatán Peninsula.
Identifiants
pubmed: 38806738
doi: 10.1007/s00248-024-02392-1
pii: 10.1007/s00248-024-02392-1
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
77Subventions
Organisme : Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México
ID : POSTDOC
Organisme : Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México
ID : POSTDOC
Organisme : FCT-Fundação para a Ciência e a Tecnologia, IP
ID : LA/P/0140/2020
Organisme : Universidad Nacional Autónoma de México, Facultad de Química
ID : PAIP 5000-9149
Organisme : Consejo Nacional de Ciencia Humanidades y Tecnología (CONAHCyT) Ciencia Básica
ID : A1-S-10785
Informations de copyright
© 2024. The Author(s).
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