Exploring the dynamic adaptive responses of Epimedium pubescens to phosphorus deficiency by Integrated transcriptome and miRNA analysis.
Epimedium pubescens
Flavonoid biosynthesis
Phosphorus deficiency
Transcriptome analysis
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
30 May 2024
30 May 2024
Historique:
received:
04
03
2024
accepted:
25
04
2024
medline:
31
5
2024
pubmed:
31
5
2024
entrez:
30
5
2024
Statut:
epublish
Résumé
Phosphorus, a crucial macronutrient essential for plant growth and development. Due to widespread phosphorus deficiency in soils, phosphorus deficiency stress has become one of the major abiotic stresses that plants encounter. Despite the evolution of adaptive mechanisms in plants to address phosphorus deficiency, the specific strategies employed by species such as Epimedium pubescens remain elusive. Therefore, this study observed the changes in the growth, physiological reponses, and active components accumulation in E. pubescensunder phosphorus deficiency treatment, and integrated transcriptome and miRNA analysis, so as to offer comprehensive insights into the adaptive mechanisms employed by E. pubescens in response to phosphorus deficiency across various stages of phosphorus treatment. Remarkably, our findings indicate that phosphorus deficiency induces root growth stimulation in E. pubescens, while concurrently inhibiting the growth of leaves, which are of medicinal value. Surprisingly, this stressful condition results in an augmented accumulation of active components in the leaves. During the early stages (30 days), leaves respond by upregulating genes associated with carbon metabolism, flavonoid biosynthesis, and hormone signaling. This adaptive response facilitates energy production, ROS scavenging, and morphological adjustments to cope with short-term phosphorus deficiency and sustain its growth. As time progresses (90 days), the expression of genes related to phosphorus cycling and recycling in leaves is upregulated, and transcriptional and post-transcriptional regulation (miRNA regulation and protein modification) is enhanced. Simultaneously, plant growth is further suppressed, and it gradually begins to discard and decompose leaves to resist the challenges of long-term phosphorus deficiency stress and sustain survival. In conclusion, our study deeply and comprehensively reveals adaptive strategies utilized by E. pubescens in response to phosphorus deficiency, demonstrating its resilience and thriving potential under stressful conditions. Furthermore, it provides valuable information on potential target genes for the cultivation of E. pubescens genotypes tolerant to low phosphorus.
Identifiants
pubmed: 38816792
doi: 10.1186/s12870-024-05063-y
pii: 10.1186/s12870-024-05063-y
doi:
Substances chimiques
Phosphorus
27YLU75U4W
MicroRNAs
0
RNA, Plant
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
480Subventions
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-031
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-031
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-031
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-031
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-031
Organisme : CAMS Innovation Fund for Medical Sciences
ID : 2021-I2M-1-031
Informations de copyright
© 2024. The Author(s).
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