Therapeutic potential activity of quercetin complexes against Streptococcus pneumoniae.
Streptococcus pneumoniae
/ drug effects
Quercetin
/ pharmacology
Anti-Bacterial Agents
/ pharmacology
Biofilms
/ drug effects
Molecular Docking Simulation
Microbial Sensitivity Tests
Drug Synergism
Bacterial Proteins
/ metabolism
Cysteine Endopeptidases
/ metabolism
Aminoacyltransferases
/ antagonists & inhibitors
Neuraminidase
/ antagonists & inhibitors
Streptococcus pneumoniae
Biofilm
Metal complexes
Quercetin
Synergism
Virulence inhibition
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 06 2024
05 06 2024
Historique:
received:
30
03
2024
accepted:
21
05
2024
medline:
5
6
2024
pubmed:
5
6
2024
entrez:
4
6
2024
Statut:
epublish
Résumé
This study investigates quercetin complexes as potential synergistic agents against the important respiratory pathogen Streptococcus pneumoniae. Six quercetin complexes (QCX1-6) were synthesized by reacting quercetin with various metal salts and boronic acids and characterized using FTIR spectroscopy. Their antibacterial activity alone and in synergism with antibiotics was evaluated against S. pneumoniae ATCC 49619 using disc diffusion screening, broth microdilution MIC determination, and checkerboard assays. Complexes QCX-3 and QCX-4 demonstrated synergy when combined with levofloxacin via fractional inhibitory concentration indices ≤ 0.5 as confirmed by time-kill kinetics. Molecular docking elucidated interactions of these combinations with virulence enzymes sortase A and sialidase. A biofilm inhibition assay found the synergistic combinations more potently reduced biofilm formation versus monotherapy. Additionally, gene-gene interaction networks, biological activity predictions and in-silico toxicity profiling provided insights into potential mechanisms of action and safety.
Identifiants
pubmed: 38834612
doi: 10.1038/s41598-024-62782-w
pii: 10.1038/s41598-024-62782-w
doi:
Substances chimiques
Quercetin
9IKM0I5T1E
Anti-Bacterial Agents
0
Bacterial Proteins
0
Cysteine Endopeptidases
EC 3.4.22.-
sortase A
EC 2.3.2.-
Aminoacyltransferases
EC 2.3.2.-
Neuraminidase
EC 3.2.1.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12876Informations de copyright
© 2024. The Author(s).
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