Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 Jun 2024
10 Jun 2024
Historique:
received:
15
08
2023
accepted:
05
06
2024
medline:
11
6
2024
pubmed:
11
6
2024
entrez:
10
6
2024
Statut:
epublish
Résumé
A crucial step in life processes is the transfer of accurate and correct genetic material to offspring. During the construction of autonomous artificial cells, a very important step is the inheritance of genetic information in divided artificial cells. The ParMRC system, as one of the most representative systems for DNA segregation in bacteria, can be purified and reconstituted into GUVs to form artificial cells. In this study, we demonstrate that the eGFP gene is segregated into two poles by a ParM filament with ParR as the intermediate linker to bind ParM and parC-eGFP DNA in artificial cells. After the ParM filament splits, the cells are externally induced to divide into two daughter cells that contain parC-eGFP DNA by osmotic pressure and laser irradiation. Using a PURE system, we translate eGFP DNA into enhanced green fluorescent proteins in daughter cells, and bacterial plasmid segregation and inheritance are successfully mimicked in artificial cells. Our results could lead to the construction of more sophisticated artificial cells that can reproduce with genetic information.
Identifiants
pubmed: 38858376
doi: 10.1038/s41467-024-49412-9
pii: 10.1038/s41467-024-49412-9
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
enhanced green fluorescent protein
0
DNA, Bacterial
0
Bacterial Proteins
0
Escherichia coli Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4956Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 22174031
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 22374033
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 21929401
Informations de copyright
© 2024. The Author(s).
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