Mechanical resistance of the environment affects root hair growth and nucleus dynamics.
Arabidopsis
thaliana
Mechanical resistance
Nucleus dynamics
Root hair
Root-soil interactions
Stiffness
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 Jun 2024
14 Jun 2024
Historique:
received:
16
02
2024
accepted:
09
06
2024
medline:
15
6
2024
pubmed:
15
6
2024
entrez:
14
6
2024
Statut:
epublish
Résumé
Root hair (RH) cells are important for the growth and survival of seedlings. They favor plant-microbe interactions and nutrients uptake. When invading the soil, RH cells have to penetrate a dense medium exhibiting a variety of physical properties, such as mechanical resistance, that impact the growth and survival of plants. Here we investigate the effect of the mechanical resistance of the culture medium on RH-physical and phenotypical parameters such as length, time, and speed of growth. We also analyze the impact of the environment on nuclear dynamics. We show that the RH growth rate and the nucleus speed decrease similarly as mechanical resistance increases while the time of growth of RH cells is invariable. Moreover, during RH growth, the nucleus-to-tip distance was found to decrease when the stiffness of the environment was increased. Along this line, using Latrunculin B treatment in liquid growth media, we could internally slow down RH growth to reach speeds similar to those observed in stiff solid media while the nucleus-to-tip distance was only slightly affected, supporting thus the idea of a specific effect of mechanical resistance of the environment on nucleus dynamics.
Identifiants
pubmed: 38877117
doi: 10.1038/s41598-024-64423-8
pii: 10.1038/s41598-024-64423-8
doi:
Substances chimiques
Culture Media
0
latrunculin B
LW7U308U7U
Thiazolidines
0
Bridged Bicyclo Compounds, Heterocyclic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
13788Subventions
Organisme : Human Frontier Science Program
ID : grant 2018, RGP, 009
Organisme : Labex Who Am I?
ID : ANR-11-LABX- 0071
Organisme : Université Paris Cité
ID : Idex ANR-18-IDEX-0001
Organisme : Agence Nationale de la Recherche
ID : Mecha-Nuc ANR-20CE13-0025-03
Informations de copyright
© 2024. The Author(s).
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