Characterization of a methyltransferase for iterative N-methylation at the leucinostatin termini in Purpureocillium lilacinum.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
22 Jun 2024
22 Jun 2024
Historique:
received:
20
08
2023
accepted:
18
06
2024
medline:
23
6
2024
pubmed:
23
6
2024
entrez:
22
6
2024
Statut:
epublish
Résumé
N-methyltransferase (NMT)-catalyzed methylation at the termini of nonribosomal peptides (NRPs) has rarely been reported. Here, we discover a fungal NMT LcsG for the iterative terminal N-methylation of a family of NRPs, leucinostatins. Gene deletion results suggest that LcsG is essential for leucinostatins methylation. Results from in vitro assays and HRESI-MS-MS analysis reveal the methylation sites as NH
Identifiants
pubmed: 38909167
doi: 10.1038/s42003-024-06467-0
pii: 10.1038/s42003-024-06467-0
doi:
Substances chimiques
Methyltransferases
EC 2.1.1.-
leucinostatin A
76600-38-9
Fungal Proteins
0
Antimicrobial Cationic Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
757Informations de copyright
© 2024. The Author(s).
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