Unveiling phenylpropanoid regulation: the role of DzMYB activator and repressor in durian (Durio zibethinus) fruit.
Plant Proteins
/ genetics
Gene Expression Regulation, Plant
Fruit
/ genetics
Transcription Factors
/ metabolism
Flavonoids
/ metabolism
Acyltransferases
/ genetics
Propanols
/ metabolism
Coenzyme A Ligases
/ metabolism
Phenols
/ metabolism
Phenylalanine Ammonia-Lyase
/ metabolism
Repressor Proteins
/ metabolism
Alcohol Oxidoreductases
/ genetics
Intramolecular Lyases
/ genetics
Basic helix–loop–helix (bHLH)
Durian
Flavonoid
MYB
Phenolic acid
Phenylpropanoid
Transcription factor
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
24 Jun 2024
24 Jun 2024
Historique:
received:
21
01
2024
accepted:
13
06
2024
medline:
24
6
2024
pubmed:
24
6
2024
entrez:
24
6
2024
Statut:
epublish
Résumé
DzMYB2 functions as an MYB activator, while DzMYB3 acts as an MYB repressor. They bind to promoters, interact with DzbHLH1, and influence phenolic contents, revealing their roles in phenylpropanoid regulation in durian pulps. Durian fruit has a high nutritional value attributed to its enriched bioactive compounds, including phenolics, carotenoids, and vitamins. While various transcription factors (TFs) regulate phenylpropanoid biosynthesis, MYB (v-myb avian myeloblastosis viral oncogene homolog) TFs have emerged as pivotal players in regulating key genes within this pathway. This study aimed to identify additional candidate MYB TFs from the transcriptome database of the Monthong cultivar at five developmental/postharvest ripening stages. Candidate transcriptional activators were discerned among MYBs upregulated during the ripe stage based on the positive correlation observed between flavonoid biosynthetic genes and flavonoid contents in ripe durian pulps. Conversely, MYBs downregulated during the ripe stage were considered candidate repressors. This study focused on a candidate MYB activator (DzMYB2) and a candidate MYB repressor (DzMYB3) for functional characterization. LC-MS/MS analysis using Nicotiana benthamiana leaves transiently expressing DzMYB2 revealed increased phenolic compound contents compared with those in leaves expressing green fluorescence protein controls, while those transiently expressing DzMYB3 showed decreased phenolic compound contents. Furthermore, it was demonstrated that DzMYB2 controls phenylpropanoid biosynthesis in durian by regulating the promoters of various biosynthetic genes, including phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR). Meanwhile, DzMYB3 regulates the promoters of PAL, 4-coumaroyl-CoA ligase (4CL), CHS, and CHI, resulting in the activation and repression of gene expression. Moreover, it was discovered that DzMYB2 and DzMYB3 could bind to another TF, DzbHLH1, in the regulation of flavonoid biosynthesis. These findings enhance our understanding of the pivotal role of MYB proteins in regulating the phenylpropanoid pathway in durian pulps.
Identifiants
pubmed: 38913159
doi: 10.1007/s00299-024-03267-y
pii: 10.1007/s00299-024-03267-y
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Flavonoids
0
Acyltransferases
EC 2.3.-
Propanols
0
Coenzyme A Ligases
EC 6.2.1.-
Phenols
0
flavanone synthetase
EC 2.3.1.74
Phenylalanine Ammonia-Lyase
EC 4.3.1.24
4-coumarate-CoA ligase
EC 6.2.1.12
Repressor Proteins
0
chalcone isomerase
EC 5.5.1.6
Alcohol Oxidoreductases
EC 1.1.-
Intramolecular Lyases
EC 5.5.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
179Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Aharoni A, De Vos CR, Wein M, Sun Z, Greco R, Kroon A, Mol JN, O’Connell AP (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J 28:319–332. https://doi.org/10.1046/j.1365-313X.2001.01154.x
doi: 10.1046/j.1365-313X.2001.01154.x
pubmed: 11722774
Albert NW, Davies KM, Lewis DH, Zhang H, Montefiori M, Brendolise C, Boase MR, Ngo H, Jameson PE, Schwinn KE (2014) A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots. Plant Cell 26:962–980. https://doi.org/10.1105/tpc.113.122069
doi: 10.1105/tpc.113.122069
pubmed: 24642943
pmcid: 4001404
Anand David A, Arulmoli R, Parasuraman S (2016) Overviews of biological importance of quercetin: a bioactive flavonoid. Pharmacogn Rev 10:84. https://doi.org/10.4103/0973-7847.194044
doi: 10.4103/0973-7847.194044
pubmed: 28082789
pmcid: 5214562
Anwar M, Yu W, Yao H, Zhou P, Allan AC, Zeng L (2019) NtMYB3, an R2R3-MYB from narcissus, regulates flavonoid biosynthesis. Int J Mol Sci 20:5456. https://doi.org/10.3390/ijms20215456
doi: 10.3390/ijms20215456
pubmed: 31683873
pmcid: 6862390
Arancibia-Avila P, Toledo F, Park YS, Jung ST, Kang SG, Heo BG, Lee SH, Sajewicz M, Kowalska T, Gorinstein S (2008) Antioxidant properties of durian fruit as influenced by ripening. LWT-Food Sci and Technol 41:2118–2125. https://doi.org/10.1016/j.lwt.2007.12.001
doi: 10.1016/j.lwt.2007.12.001
Aziz NAA, Jalil AM (2019) Bioactive compounds, nutritional value, and potential health benefits of indigenous durian (Durio zibethinus Murr.): a review. Foods of Plant Ori. https://doi.org/10.3390/foods8030096
Bovy A, de Vos R, Kemper M, Schijlen E, Almenar PM, Muir S, Collins G, Robinson S, Verhoeyen M, Hughes S (2002) High-flavonol tomatoes resulting from the heterologous expression of the maize transcription factor genes LC and C1. Plant Cell 14:2509–2526. https://doi.org/10.1105/tpc.004218
doi: 10.1105/tpc.004218
pubmed: 12368501
pmcid: 151232
Brodowska KM (2017) Natural flavonoids: classification, potential role, and application of flavonoid analogues. Eur J Biol Res 7:108–123. https://doi.org/10.4161/psb.5.6.11570
doi: 10.4161/psb.5.6.11570
Colquhoun TA, Kim JY, Wedde AE, Levin LA, Schmitt KC, Schuurink RC, Clark DG (2011) PhMYB4 fine-tunes the floral volatile signature of Petunia× hybrida through PhC4H. J Exp Bot 62:1133–1143. https://doi.org/10.1093/jxb/erq342
doi: 10.1093/jxb/erq342
pubmed: 21068208
Deng Y, Lu S (2017) Biosynthesis and regulation of phenylpropanoids in plants. Crit Rev Plant Sci 36:257–290. https://doi.org/10.1080/07352689.2017.1402852
doi: 10.1080/07352689.2017.1402852
Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trends Plant Sci 15:573–581. https://doi.org/10.1016/j.tplants.2010.06.005
doi: 10.1016/j.tplants.2010.06.005
pubmed: 20674465
Falcone Ferreyra ML, Rius SP, Casati P (2012) Flavonoids: biosynthesis, biological functions, and biotechnological applications. Front Plant Sci 3:222. https://doi.org/10.3389/fpls.2012.00222
doi: 10.3389/fpls.2012.00222
pubmed: 23060891
pmcid: 3460232
Fan Y, Peng J, Wu J, Zhou P, He R, Allan AC, Zeng L (2021) NtbHLH1, a JAF13-like bHLH, interacts with NtMYB6 to enhance proanthocyanidin accumulation in Chinese Narcissus. BMC Plant Biol 21:1–14. https://doi.org/10.1186/s12870-021-03050-1
doi: 10.1186/s12870-021-03050-1
Fornalé S, Shi X, Chai C, Encina A, Irar S, Capellades M, Fuguet E, Torres JL, Rovira P, Puigdomenech P (2010) ZmMYB31 directly represses maize lignin genes and redirects the phenylpropanoid metabolic flux. Plant J 64:633–644. https://doi.org/10.1111/j.1365-313X.2010.04363.x
doi: 10.1111/j.1365-313X.2010.04363.x
pubmed: 21070416
Gorinstein S, Poovarodom S, Leontowicz H, Leontowicz M, Namiesnik J, Vearasilp S, Haruenkit R, Ruamsuke P, Katrich E, Tashma Z (2011) Antioxidant properties and bioactive constituents of some rare exotic Thai fruits and comparison with conventional fruits: In vitro and in vivo studies. Food Res Int 44:2222–2232. https://doi.org/10.1016/j.foodres.2010.10.009
doi: 10.1016/j.foodres.2010.10.009
Hellens RP, Allan AC, Friel EN, Bolitho K, Grafton K, Templeton MD, Karunairetnam S, Gleave AP, Laing WA (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1:1–14. https://doi.org/10.1186/1746-4811-1-13
doi: 10.1186/1746-4811-1-13
Hichri I, Heppel SC, Pillet J, Léon C, Czemmel S, Delrot S, Lauvergeat V, Bogs J (2010) The basic helix-loop-helix transcription factor MYC1 is involved in the regulation of the flavonoid biosynthesis pathway in grapevine. Mol Plant 3:509–523. https://doi.org/10.1093/mp/ssp118
doi: 10.1093/mp/ssp118
pubmed: 20118183
Kagale S, Rozwadowski K (2011) EAR motif-mediated transcriptional repression in plants: an underlying mechanism for epigenetic regulation of gene expression. Epigenetics 6:141–146. https://doi.org/10.4161/epi.6.2.13627
doi: 10.4161/epi.6.2.13627
pubmed: 20935498
pmcid: 3278782
Khaksar G, Sangchay W, Pinsorn P, Sangpong L, Sirikantaramas S (2019) Genome-wide analysis of the Dof gene family in durian reveals fruit ripening-associated and cultivar-dependent Dof transcription factors. Sci Rep 9:12109. https://doi.org/10.1038/s41598-019-48601-7
doi: 10.1038/s41598-019-48601-7
pubmed: 31431665
pmcid: 6702166
Khaksar G, Kasemcholathan S, Sirikantaramas S (2024) Durian (Durio zibethinus L.): nutritional composition, pharmacological implications, value-added products, and omics-based investigations. Hortic 10:342. https://doi.org/10.3390/horticulturae10040342
doi: 10.3390/horticulturae10040342
Klempnauer KH, Gonda TJ, Bishop JM (1982) Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: the architecture of a transduced oncogene. Cell 31:453–463. https://doi.org/10.1016/0092-8674(82)90138-6
doi: 10.1016/0092-8674(82)90138-6
pubmed: 6297766
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547. https://doi.org/10.1093/molbev/msy096
doi: 10.1093/molbev/msy096
pubmed: 29722887
pmcid: 5967553
Lai Z, Tsugawa H, Wohlgemuth G, Mehta S, Mueller M, Zheng Y, Ogiwara A, Meissen J, Showalter M, Takeuchi K (2018) Identifying metabolites by integrating metabolome databases with mass spectrometry cheminformatics. Nat Methods 15:53–56. https://doi.org/10.1038/nmeth.4512
doi: 10.1038/nmeth.4512
pubmed: 29176591
Li Y, Zhang T, Chen GY (2018) Flavonoids and colorectal cancer prevention. Antioxidants 7:187. https://doi.org/10.3390/antiox7120187
doi: 10.3390/antiox7120187
pubmed: 30544686
pmcid: 6316869
Lindbo JA (2007) High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virus expression vectors. BMC Biotechnol 7:1–11. https://doi.org/10.1186/1472-6750-7-52
doi: 10.1186/1472-6750-7-52
Liu J, Osbourn A, Ma P (2015) MYB transcription factors as regulators of phenylpropanoid metabolism in plants. Mol Plant 8:689–708. https://doi.org/10.1016/j.molp.2015.03.012
doi: 10.1016/j.molp.2015.03.012
pubmed: 25840349
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2
doi: 10.1006/meth.2001.1262
pubmed: 11846609
Luo J, Butelli E, Hill L, Parr A, Niggeweg R, Bailey P, Weisshaar B, Martin C (2008) AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: expression in fruit results in very high levels of both types of polyphenol. Plant J 56:316–326. https://doi.org/10.1111/j.1365-313X.2008.03597.x
doi: 10.1111/j.1365-313X.2008.03597.x
pubmed: 18643978
Ma D, Constabel CP (2019) MYB repressors as regulators of phenylpropanoid metabolism in plants. Trends Plant Sci 24:275–289. https://doi.org/10.1016/j.tplants.2018.12.003
doi: 10.1016/j.tplants.2018.12.003
pubmed: 30704824
Ma D, Sun D, Wang C, Li Y, Guo T (2014) Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress. Plant Physiol Biochem 80:60–66. https://doi.org/10.1016/j.plaphy.2014.03.024
doi: 10.1016/j.plaphy.2014.03.024
pubmed: 24727789
Massari ME, Murre C (2000) Helix-loop-helix proteins: regulators of transcription in eucaryotic organisms. Mol Cell Biol 20:429–440. https://doi.org/10.1128/MCB.20.2.429-440.2000
doi: 10.1128/MCB.20.2.429-440.2000
pubmed: 10611221
pmcid: 85097
Mattila P, Hellström J (2007) Phenolic acids in potatoes, vegetables, and some of their products. J Food Compost Anal 20:152–160. https://doi.org/10.1016/j.jfca.2006.05.007
doi: 10.1016/j.jfca.2006.05.007
Mehrtens F, Kranz H, Bednarek P, Weisshaar B (2005) The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol 138:1083–1096. https://doi.org/10.1104/pp.104.058032
doi: 10.1104/pp.104.058032
pubmed: 15923334
pmcid: 1150422
Miller JC, Chezem WR, Clay NK (2016) Ternary WD40 repeat-containing protein complexes: evolution, composition and roles in plant immunity. Front Plant Sci 6:1108. https://doi.org/10.3389/fpls.2015.01108
doi: 10.3389/fpls.2015.01108
pubmed: 26779203
pmcid: 4703829
Nakagawa T, Kurose T, Hino T, Tanaka K, Kawamukai M, Niwa Y, Toyooka K, Matsuoka K, Jinbo T, Kimura T (2007) Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation. J Biosci Bioeng 104:34–41. https://doi.org/10.1263/jbb.104.34
doi: 10.1263/jbb.104.34
pubmed: 17697981
Nemesio-Gorriz M, Blair PB, Dalman K, Hammerbacher A, Arnerup J, Stenlid J, Mukhtar SM, Elfstrand M (2017) Identification of Norway spruce MYB-bHLH-WDR transcription factor complex members linked to regulation of the flavonoid pathway. Front Plant Sci 8:305. https://doi.org/10.3389/fpls.2017.00305
doi: 10.3389/fpls.2017.00305
pubmed: 28337212
pmcid: 5343035
Pandey A, Misra P, Chandrashekar K, Trivedi PK (2012) Development of AtMYB12-expressing transgenic tobacco callus culture for production of rutin with biopesticidal potential. Plant Cell Rep 31:1867–1876. https://doi.org/10.1007/s00299-012-1300-6
doi: 10.1007/s00299-012-1300-6
pubmed: 22733206
Pandey A, Misra P, Khan MP, Swarnkar G, Tewari MC, Bhambhani S, Trivedi R, Chattopadhyay N, Trivedi PK (2014) Co-expression of Arabidopsis transcription factor, At MYB12, and soybean isoflavone synthase, Gm IFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. Plant Biotechnol J 12:69–80. https://doi.org/10.1111/pbi.12118
doi: 10.1111/pbi.12118
pubmed: 24102754
Pang Z, Chong J, Zhou G, de Lima Morais DA, Chang L, Barrette M, Gauthier C, Jacques PÉ, Li S, Xia J (2021) MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights. Nucleic Acids Res 49:W388–W396. https://doi.org/10.1093/nar/gkab382
doi: 10.1093/nar/gkab382
pubmed: 34019663
pmcid: 8265181
Payyavula RS, Singh RK, Navarre DA (2013) Transcription factors, sucrose, and sucrose metabolic genes interact to regulate potato phenylpropanoid metabolism. J Exp Bot 64:5115–5131. https://doi.org/10.1093/jxb/ert303
doi: 10.1093/jxb/ert303
pubmed: 24098049
pmcid: 3830490
Paz-Ares J, Ghosal D, Wienand U, Peterson P, Saedler H (1987) The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J 6:3553–3558. https://doi.org/10.1002/j.1460-2075.1987.tb02684.x
doi: 10.1002/j.1460-2075.1987.tb02684.x
pubmed: 3428265
pmcid: 553820
Persak H, Pitzschke A (2013) Tight interconnection and multi-level control of Arabidopsis MYB44 in MAPK cascade signalling. PLoS ONE 8:e57547. https://doi.org/10.1371/journal.pone.0057547
doi: 10.1371/journal.pone.0057547
pubmed: 23437396
pmcid: 3578790
Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15:8–15. https://doi.org/10.1007/BF02772108
doi: 10.1007/BF02772108
Pratyusha DS, Sarada DV (2022) MYB transcription factors—master regulators of phenylpropanoid biosynthesis and diverse developmental and stress responses. Plant Cell Rep 41:2245–2260. https://doi.org/10.1007/s00299-022-02927-1
doi: 10.1007/s00299-022-02927-1
pubmed: 36171500
Rommens CM, Richael CM, Yan H, Navarre DA, Ye J, Krucker M, Swords K (2008) Engineered native pathways for high kaempferol and caffeoylquinate production in potato. Plant Biotechnol J 6:870–886. https://doi.org/10.1111/j.1467-7652.2008.00362.x
doi: 10.1111/j.1467-7652.2008.00362.x
pubmed: 18662373
Shen H, He X, Poovaiah CR, Wuddineh WA, Ma J, Mann DG, Wang H, Jackson L, Tang Y, Stewart N, CJr. (2012) Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks. New Phytol 193:121–136. https://doi.org/10.1111/j.1469-8137.2011.03922.x
doi: 10.1111/j.1469-8137.2011.03922.x
pubmed: 21988539
Stracke R, Ishihara H, Huep G, Barsch A, Mehrtens F, Niehaus K, Weisshaar B (2007) Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling. Plant J 50:660–677. https://doi.org/10.1111/j.1365-313X.2007.03078.x
doi: 10.1111/j.1365-313X.2007.03078.x
pubmed: 17419845
pmcid: 1976380
Suntichaikamolkul N, Sangpong L, Schaller H, Sirikantaramas S (2021) Genome-wide identification and expression profiling of durian CYPome related to fruit ripening. PLoS ONE 16:e0260665. https://doi.org/10.1371/journal.pone.0260665
doi: 10.1371/journal.pone.0260665
pubmed: 34847184
pmcid: 8631664
Tamagnone L, Merida A, Parr A, Mackay S, Culianez-Macia FA, Roberts K, Martin C (1998) The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco. Plant Cell 10:135–154. https://doi.org/10.1105/tpc.10.2.135
doi: 10.1105/tpc.10.2.135
pubmed: 9490739
pmcid: 143979
Toledo F, Arancibia-Avila P, Park YS, Jung ST, Kang SG, Gu HB, Drzewiecki J, Zachwieja Z, Zagrodzki P, Pasko P (2008) Screening of the antioxidant and nutritional properties, phenolic contents and proteins of five durian cultivars. Int J Food Sci Nutr INT J FOOD SCI NUTR 59:415–427. https://doi.org/10.1080/09637480701603082
doi: 10.1080/09637480701603082
pubmed: 18979619
Wang D, Song Y, Chen Y, Yao W, Li Z, Liu W, Yue S, Wang Z (2013) Metabolic pools of phenolic acids in Salvia miltiorrhiza are enhanced by co-expression of Antirrhinum majus Delila and Rosea1 transcription factors. Biochem Eng J 74:115–120. https://doi.org/10.1016/j.bej.2013.02.014
doi: 10.1016/j.bej.2013.02.014
Wang D, Fan W, Guo X, Wu K, Zhou S, Chen Z, Li D, Wang K, Zhu Y, Zhou Y (2020) MaGenDB: a functional genomics hub for Malvaceae plants. Nucleic Acids Res 48:D1076–D1084. https://doi.org/10.1093/nar/gkz953
doi: 10.1093/nar/gkz953
pubmed: 31665439
Weerawanich K, Halbwirth H, Sirikantaramas S (2024) A novel MYB transcription factor from durian (Durio zibethinus), DzMYB1, regulates flavonoid biosynthesis in fruit pulp. Sci Hortic 333:113246. https://doi.org/10.1016/j.scienta.2024.113246
doi: 10.1016/j.scienta.2024.113246
Wei L, Mao W, Jia M, Xing S, Ali U, Zhao Y, Chen Y, Cao M, Dai Z, Zhang K (2018) FaMYB44. 2, a transcriptional repressor, negatively regulates sucrose accumulation in strawberry receptacles through interplay with FaMYB10. J Exp Bot 69:4805–4820. https://doi.org/10.1093/jxb/ery249
doi: 10.1093/jxb/ery249
pubmed: 30085079
pmcid: 6137983
Xu W, Dubos C, Lepiniec L (2015) Transcriptional control of flavonoid biosynthesis by MYB–bHLH–WDR complexes. Trends Plant Sci 20:176–185. https://doi.org/10.1016/j.tplants.2014.12.001
doi: 10.1016/j.tplants.2014.12.001
pubmed: 25577424
Xu H, Wang N, Liu J, Qu C, Wang Y, Jiang S, Lu N, Wang D, Zhang Z, Chen X (2017) The molecular mechanism underlying anthocyanin metabolism in apple using the MdMYB16 and MdbHLH33 genes. Plant Mol Biol 94:149–165. https://doi.org/10.1007/s11103-017-0601-0
doi: 10.1007/s11103-017-0601-0
pubmed: 28286910
Yoo SD, Cho YH, Sheen J (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2:1565–1572. https://doi.org/10.1038/nprot.2007.199
doi: 10.1038/nprot.2007.199
pubmed: 17585298
Yue M, Jiang L, Zhang N, Zhang L, Liu Y, Lin Y, Zhang Y, Luo Y, Zhang Y, Wang Y (2023) Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes. Front Plant Sci 14:1145670. https://doi.org/10.3389/fpls.2023.1145670
doi: 10.3389/fpls.2023.1145670
pubmed: 36993840
pmcid: 10040760
Zhang S, Ma P, Yang D, Li W, Liang Z, Liu Y, Liu F (2013) Cloning and characterization of a putative R2R3 MYB transcriptional repressor of the rosmarinic acid biosynthetic pathway from Salvia miltiorrhiza. PLoS ONE 8:e73259. https://doi.org/10.1371/journal.pone.0073259
doi: 10.1371/journal.pone.0073259
pubmed: 24039895
pmcid: 3769309
Zhang H, Gong J, Chen K, Yao W, Zhang B, Wang J, Tian S, Liu H, Wang Y, Liu Y (2020) A novel R3 MYB transcriptional repressor, MaMYBx, finely regulates anthocyanin biosynthesis in grape hyacinth. Plant Sci 298:110588. https://doi.org/10.1016/j.plantsci.2020.110588
doi: 10.1016/j.plantsci.2020.110588
pubmed: 32771147
Zimmermann IM, Heim MA, Weisshaar B, Uhrig JF (2004) Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins. Plant J 40:22–34. https://doi.org/10.1111/j.1365-313X.2004.02183.x
doi: 10.1111/j.1365-313X.2004.02183.x
pubmed: 15361138