Multi-genome comparisons reveal gain-and-loss evolution of anti-Mullerian hormone receptor type 2 as a candidate master sex-determining gene in Percidae.

Animals Sex Determination Processes / genetics Evolution, Molecular Male Female Perches / genetics Phylogeny Receptors, Peptide / genetics Genome Receptors, Transforming Growth Factor beta

Genome Perches Pikeperches Sex chromosomes Sex-determination

Journal

BMC biology

ISSN: 1741-7007

Titre abrégé: BMC Biol

Pays: England

ID NLM: 101190720

Informations de publication

Date de publication:
26 Jun 2024

Historique:

received: 19 01 2024

accepted: 06 06 2024

medline: 27 6 2024

pubmed: 27 6 2024

entrez: 26 6 2024

Statut: epublish

Résumé

The Percidae family comprises many fish species of major importance for aquaculture and fisheries. Based on three new chromosome-scale assemblies in Perca fluviatilis, Perca schrenkii, and Sander vitreus along with additional percid fish reference genomes, we provide an evolutionary and comparative genomic analysis of their sex-determination systems. We explored the fate of a duplicated anti-Mullerian hormone receptor type-2 gene (amhr2bY), previously suggested to be the master sex-determining (MSD) gene in P. flavescens. Phylogenetically related and structurally similar amhr2 duplicates (amhr2b) were found in P. schrenkii and Sander lucioperca, potentially dating this duplication event to their last common ancestor around 19-27 Mya. In P. fluviatilis and S. vitreus, this amhr2b duplicate has been likely lost while it was subject to amplification in S. lucioperca. Analyses of the amhr2b locus in P. schrenkii suggest that this duplication could be also male-specific as it is in P. flavescens. In P. fluviatilis, a relatively small (100 kb) non-recombinant sex-determining region (SDR) was characterized on chromosome 18 using population-genomics approaches. This SDR is characterized by many male-specific single-nucleotide variations (SNVs) and no large duplication/insertion event, suggesting that P. fluviatilis has a male heterogametic sex-determination system (XX/XY), generated by allelic diversification. This SDR contains six annotated genes, including three (c18h1orf198, hsdl1, tbc1d32) with higher expression in the testis than in the ovary. Together, our results provide a new example of the highly dynamic sex chromosome turnover in teleosts and provide new genomic resources for Percidae, including sex-genotyping tools for all three known Perca species.

Sections du résumé

BACKGROUND BACKGROUND

RESULTS RESULTS

We explored the fate of a duplicated anti-Mullerian hormone receptor type-2 gene (amhr2bY), previously suggested to be the master sex-determining (MSD) gene in P. flavescens. Phylogenetically related and structurally similar amhr2 duplicates (amhr2b) were found in P. schrenkii and Sander lucioperca, potentially dating this duplication event to their last common ancestor around 19-27 Mya. In P. fluviatilis and S. vitreus, this amhr2b duplicate has been likely lost while it was subject to amplification in S. lucioperca. Analyses of the amhr2b locus in P. schrenkii suggest that this duplication could be also male-specific as it is in P. flavescens. In P. fluviatilis, a relatively small (100 kb) non-recombinant sex-determining region (SDR) was characterized on chromosome 18 using population-genomics approaches. This SDR is characterized by many male-specific single-nucleotide variations (SNVs) and no large duplication/insertion event, suggesting that P. fluviatilis has a male heterogametic sex-determination system (XX/XY), generated by allelic diversification. This SDR contains six annotated genes, including three (c18h1orf198, hsdl1, tbc1d32) with higher expression in the testis than in the ovary.

CONCLUSIONS CONCLUSIONS

Together, our results provide a new example of the highly dynamic sex chromosome turnover in teleosts and provide new genomic resources for Percidae, including sex-genotyping tools for all three known Perca species.

Identifiants

DOI: 10.1186/s12915-024-01935-9 PMID: 38926709

pubmed: 38926709

doi: 10.1186/s12915-024-01935-9

pii: 10.1186/s12915-024-01935-9

doi:

Substances chimiques

anti-Mullerian hormone receptor 0

Receptors, Peptide 0

Receptors, Transforming Growth Factor beta 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

141

Subventions

Organisme : Agence Nationale de la Recherche

ID : ANR-13-ISV7-0005

Organisme : Agence Nationale de la Recherche

ID : ANR-10-INBS-09

Organisme : Deutsche Forschungsgemeinschaft

ID : KU 3596/1-1

Organisme : Deutsche Forschungsgemeinschaft

ID : project number: 324050651

Informations de copyright

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