In silico studies and development of a protein-based electrochemical sensor for selective and sensitive detection of aflatoxin B1.


Journal

Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782

Informations de publication

Date de publication:
27 Jun 2024
Historique:
received: 04 03 2024
accepted: 07 06 2024
medline: 27 6 2024
pubmed: 27 6 2024
entrez: 27 6 2024
Statut: epublish

Résumé

Proteins from different species have been docked with aflatoxin B1 (AFB1) and identified 3 proteins (prostaglandin-E(2)9-reductase from Oryctolagus uniculus, proto-oncogene serine/threonine-protein kinase Pim-1 and human immunoglobulin G (hIgG)) as potential candidates to develop an electrochemical sensor. Fluorescence spectroscopy experiments have confirmed the interaction of hIgG with AFB1 with an affinity constant of 4.6 × 10

Identifiants

pubmed: 38935329
doi: 10.1007/s00604-024-06495-x
pii: 10.1007/s00604-024-06495-x
doi:

Substances chimiques

Aflatoxin B1 9N2N2Y55MH
Nanotubes, Carbon 0
Immunoglobulin G 0
Proto-Oncogene Mas 0
MAS1 protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

426

Subventions

Organisme : Department of Biotechnology (DBT)
ID : BT/PR29096/PFN/20/1371/2018
Organisme : VIT University
ID : SG20220096
Organisme : Indian Council of Medical Research
ID : 17x(3)/Adhoc/32/2022-ITR

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

Références

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Auteurs

Megala Ulaganambi (M)

Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, 632014, Tamilnadu, India.

Lokesh Kumar S (LK)

Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, 632014, Tamilnadu, India.

Sanjit Kumar (S)

Department of Biotechnology, School of Interdisciplinary Education and Research, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, 495009, India.

Kishore K R Tetala (KKR)

Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, 632014, Tamilnadu, India. kishore.tetala@gmail.com.

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