Bacterial exonuclease III expands its enzymatic activities on single-stranded DNA.

Exodeoxyribonucleases / metabolism DNA, Single-Stranded / metabolism Escherichia coli / genetics Escherichia coli Proteins / metabolism
E. coli ExoⅢ-based diagnostics biochemistry chemical biology endonuclease activity exonuclease exonuclease Ⅲ fluorescence-quenching reporter

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
03 Jul 2024
Historique:
medline: 3 7 2024
pubmed: 3 7 2024
entrez: 3 7 2024
Statut: epublish

Résumé

Bacterial exonuclease III (ExoIII), widely acknowledged for specifically targeting double-stranded DNA (dsDNA), has been documented as a DNA repair-associated nuclease with apurinic/apyrimidinic (AP)-endonuclease and 3'→5' exonuclease activities. Due to these enzymatic properties, ExoIII has been broadly applied in molecular biosensors. Here, we demonstrate that ExoIII (

Identifiants

DOI: 10.7554/eLife.95648 PMID: 38959062
pubmed: 38959062
doi: 10.7554/eLife.95648
pii: 95648
doi:
pii:

Substances chimiques

Exodeoxyribonucleases EC 3.1.-
DNA, Single-Stranded 0
exodeoxyribonuclease III EC 3.1.11.2
Escherichia coli Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Zhejiang Provincial Key R&D Program
ID : 2023C03045
Organisme : National Program on Key Research Project of China
ID : 2022YFC2604201
Organisme : Horizon 2020 - Research and Innovation Framework Programme
ID : 861917-SAFFI
Organisme : Zhejiang Provincial Natural Science Foundation of China
ID : LZ24C180002
Organisme : Scientific Research Fund of Zhejiang Provincial Education Department
ID : Y202250872
Organisme : Key research and Development Program of Hangzhou
ID : 202203A08
Organisme : National Program on Key Research Project of China
ID : 2019YFE0103900
Organisme : Zhejiang Provincial Key R&D Program
ID : 2021C02008

Informations de copyright

© 2024, Wang et al.

Déclaration de conflit d'intérêts

HW, CY, QL, ZJ, CJ, CZ, NL, CZ, GZ, MY, YL No competing interests declared

Auteurs

Hao Wang (H)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.

Chen Ye (C)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.

Qi Lu (Q)

Hainan Institute of Zhejiang University, Sanya, China.

Zhijie Jiang (Z)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.

Chao Jiang (C)

Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang, China.

Chun Zhou (C)

School of Public Health, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
ORCID: 0000-0002-9257-468X

Na Li (N)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.

Caiqiao Zhang (C)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.
ORCID: 0000-0002-3519-9457

Guoping Zhao (G)

Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.

Min Yue (M)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.
Hainan Institute of Zhejiang University, Sanya, China.
Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
ORCID: 0000-0002-6787-0794

Yan Li (Y)

Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China.
Hainan Institute of Zhejiang University, Sanya, China.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Exonucleases › Exodeoxyribonucleases › Bacterial exonuclease III expands its...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ADN simple brin › Bacterial exonuclease III expands its...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Bacterial exonuclease III expands its...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Bacterial exonuclease III expands its...