Metabolic engineering of Komagataella phaffii for the efficient utilization of methanol.
Komagataella Phaffii
Formaldehyde
Methanol
NAD+/NADH
Xu5P
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
17 Jul 2024
17 Jul 2024
Historique:
received:
25
04
2024
accepted:
08
07
2024
medline:
17
7
2024
pubmed:
17
7
2024
entrez:
16
7
2024
Statut:
epublish
Résumé
Komagataella phaffii, a type of methanotrophic yeast, can use methanol, a favorable non-sugar substrate in eco-friendly bio-manufacturing. The dissimilation pathway in K. phaffii leads to the loss of carbon atoms in the form of CO Inhibiting the dissimilation pathway triggers an excessive accumulation of formaldehyde and a decline in the intracellular NAD The reduction of formaldehyde accumulation, the increase of NAD
Sections du résumé
BACKGROUND
BACKGROUND
Komagataella phaffii, a type of methanotrophic yeast, can use methanol, a favorable non-sugar substrate in eco-friendly bio-manufacturing. The dissimilation pathway in K. phaffii leads to the loss of carbon atoms in the form of CO
RESULTS
RESULTS
Inhibiting the dissimilation pathway triggers an excessive accumulation of formaldehyde and a decline in the intracellular NAD
CONCLUSIONS
CONCLUSIONS
The reduction of formaldehyde accumulation, the increase of NAD
Identifiants
pubmed: 39014373
doi: 10.1186/s12934-024-02475-1
pii: 10.1186/s12934-024-02475-1
doi:
Substances chimiques
Methanol
Y4S76JWI15
Alcohol Oxidoreductases
EC 1.1.-
Formaldehyde
1HG84L3525
formaldehyde dehydrogenase, glutathione-independent
EC 1.2.1.46
Aldehyde Oxidoreductases
EC 1.2.-
NAD
0U46U6E8UK
alcohol oxidase
EC 1.1.3.13
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
198Subventions
Organisme : National Key Research and Development Program of China
ID : 2022YFC2105501
Informations de copyright
© 2024. The Author(s).
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