1-Butanol treatment enhances drought stress tolerance in Arabidopsis thaliana.


Journal

Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343

Informations de publication

Date de publication:
18 Jul 2024
Historique:
received: 08 01 2024
accepted: 14 06 2024
medline: 18 7 2024
pubmed: 18 7 2024
entrez: 18 7 2024
Statut: epublish

Résumé

Abiotic stress is a major factor affecting crop productivity. Chemical priming is a promising strategy to enhance tolerance to abiotic stress. In this study, we evaluated the use of 1-butanol as an effectual strategy to enhance drought stress tolerance in Arabidopsis thaliana. We first demonstrated that, among isopropanol, methanol, 1-butanol, and 2-butanol, pretreatment with 1-butanol was the most effective for enhancing drought tolerance. We tested the plants with a range of 1-butanol concentrations (0, 10, 20, 30, 40, and 50 mM) and further determined that 20 mM was the optimal concentration of 1-butanol that enhanced drought tolerance without compromising plant growth. Physiological tests showed that the enhancement of drought tolerance by 1-butanol pretreatment was associated with its stimulation of stomatal closure and improvement of leaf water retention. RNA-sequencing analysis revealed the differentially expressed genes (DEGs) between water- and 1-butanol-pretreated plants. The DEGs included genes involved in oxidative stress response processes. The DEGs identified here partially overlapped with those of ethanol-treated plants. Taken together, the results show that 1-butanol is a novel chemical priming agent that effectively enhances drought stress tolerance in Arabidopsis plants, and provide insights into the molecular mechanisms of alcohol-mediated abiotic stress tolerance.

Identifiants

pubmed: 39023668
doi: 10.1007/s11103-024-01479-0
pii: 10.1007/s11103-024-01479-0
doi:

Substances chimiques

1-Butanol 8PJ61P6TS3
Water 059QF0KO0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

86

Subventions

Organisme : RIKEN-AIST Joint Research Fund (full research), Core Research for Evolutionary Science and Technology
ID : JPMJCR13B4
Organisme : A-STEP of the Japan Science and Technology Agency (JST)
ID : JPMJTM19BS
Organisme : Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : Innovative Areas 18H04791
Organisme : Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : 18H04705

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Thi Nhu Quynh Do (TNQ)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan.
National Key Laboratory for Plant Cell Biotechnology, Agricultural Genetics Institute, Pham Van Dong Road, Bac Tu Liem District, Hanoi, Vietnam.

Daisuke Todaka (D)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan.

Maho Tanaka (M)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan.
Plant Epigenome Regulation Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.

Satoshi Takahashi (S)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan.
Plant Epigenome Regulation Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.

Junko Ishida (J)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan.
Plant Epigenome Regulation Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.

Kaori Sako (K)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan.
Department of Advanced Bioscience, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan.

Atsushi J Nagano (AJ)

Faculty of Agriculture, Ryukoku University, Shiga, 520-2194, Japan.
Institute for Advanced Biosciences, Keio University, Yamagata, 997-0017, Japan.

Yumiko Takebayashi (Y)

Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.

Yuri Kanno (Y)

Plant Chemical Genomics Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.

Masanori Okamoto (M)

Plant Chemical Genomics Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.

Xuan Hoi Pham (XH)

National Key Laboratory for Plant Cell Biotechnology, Agricultural Genetics Institute, Pham Van Dong Road, Bac Tu Liem District, Hanoi, Vietnam.

Motoaki Seki (M)

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-Cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan. motoaki.seki@riken.jp.
Plant Epigenome Regulation Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan. motoaki.seki@riken.jp.
Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-Cho, Totsuka-Ku, Yokohama, Kanagawa, 244-0813, Japan. motoaki.seki@riken.jp.
Graduate School of Science and Engineering, Saitama University, Saitama, Saitama, 338-8570, Japan. motoaki.seki@riken.jp.

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