Atomic Force Microscopy Characterization of Reconstituted Protein-DNA Complexes.
Archaea
Atomic force microscopy
Chromatin
Chromatin reconstitution
Nucleoid
Nucleoid-associated protein (NAP)
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
19
7
2024
pubmed:
19
7
2024
entrez:
19
7
2024
Statut:
ppublish
Résumé
Atomic force microscopy is a high-resolution imaging technique useful for observing the structures of biomolecular complexes. This approach provides a straightforward method to characterize the binding behavior of different chromatin architectural proteins and to analyze the increasingly complex structural units assembled on the DNA. The protocol describes the preparation, AFM imaging, and structural analysis of chromatin that is reconstituted in vitro using purified proteins and DNA. Here, we describe the successful application of the method on the chromatin architectural proteins of the archaeon Sulfolobus solfataricus.
Identifiants
pubmed: 39028512
doi: 10.1007/978-1-0716-3930-6_14
doi:
Substances chimiques
DNA
9007-49-2
Chromatin
0
DNA-Binding Proteins
0
Archaeal Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
279-295Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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