Fast screening of α-glucosidase inhibitors from Ginkgo biloba leaf by using α-glucosidase immobilized on magnetic metal-organic framework.
Ginkgo biloba
/ chemistry
Glycoside Hydrolase Inhibitors
/ chemistry
Metal-Organic Frameworks
/ chemistry
Plant Leaves
/ chemistry
alpha-Glucosidases
/ metabolism
Enzymes, Immobilized
/ chemistry
Molecular Docking Simulation
Drug Evaluation, Preclinical
Plant Extracts
/ chemistry
Quercetin
/ chemistry
Chromatography, High Pressure Liquid
Ginkgo biloba leaf
inhibitor screening
magnetic metal‐organic framework
molecular docking
α‐glucosidase
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
revised:
21
06
2024
received:
09
05
2024
accepted:
28
06
2024
medline:
20
7
2024
pubmed:
20
7
2024
entrez:
20
7
2024
Statut:
ppublish
Résumé
In this study, a ligand fishing method for the screening of α-glucosidase inhibitors from Ginkgo biloba leaf was established for the first time using α-glucosidase immobilized on the magnetic metal-organic framework. The immobilized α-glucosidase exhibited enhanced resistance to temperature and pH, as well as good thermal stability and reusability. Two ligands, namely quercitrin and quercetin, were screened from Ginkgo biloba leaf and identified by ultra-high performance liquid chromatography-tandem mass spectrometry. The half-maximal inhibitory concentration values for quercitrin and quercetin were determined to be 105.69 ± 0.39 and 83.49 ± 0.79 µM, respectively. Molecular docking further confirmed the strong inhibitory effect of these two ligands. The proposed approach in this study demonstrates exceptional efficiency in the screening of α-glucosidase inhibitors from complex natural medicinal plants, thus exhibiting significant potential for the discovery of antidiabetic compounds.
Identifiants
pubmed: 39031453
doi: 10.1002/jssc.202400342
doi:
Substances chimiques
Glycoside Hydrolase Inhibitors
0
Metal-Organic Frameworks
0
alpha-Glucosidases
EC 3.2.1.20
Enzymes, Immobilized
0
Plant Extracts
0
Quercetin
9IKM0I5T1E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2400342Subventions
Organisme : Foundation of the Chinese Academy of Sciences
Organisme : Science and Technology Programs of Sichuan Province of China
ID : 2021YFS0060
Organisme : West Light Foundation, Chinese Academy of Sciences
Informations de copyright
© 2024 Wiley‐VCH GmbH.
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