Single-nucleus RNA sequencing reveals glial cell type-specific responses to ischemic stroke in male rodents.

Animals Ischemic Stroke / genetics Male Mice Single-Cell Analysis / methods Oligodendroglia / metabolism Oligodendrocyte Precursor Cells / metabolism Astrocytes / metabolism Neuroglia / metabolism Osteopontin / genetics Transcriptome Sequence Analysis, RNA / methods Mice, Inbred C57BL Brain / metabolism Rats Cell Proliferation Cell Movement / genetics Myeloid Cells / metabolism Disease Models, Animal Cell Nucleus / metabolism Brain Ischemia / genetics

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
24 Jul 2024

Historique:

received: 08 01 2024

accepted: 09 07 2024

medline: 24 7 2024

pubmed: 24 7 2024

entrez: 23 7 2024

Statut: epublish

Résumé

Neuroglia critically shape the brain´s response to ischemic stroke. However, their phenotypic heterogeneity impedes a holistic understanding of the cellular composition of the early ischemic lesion. Here we present a single cell resolution transcriptomics dataset of the brain´s acute response to infarction. Oligodendrocyte lineage cells and astrocytes range among the most transcriptionally perturbed populations and exhibit infarction- and subtype-specific molecular signatures. Specifically, we find infarction restricted proliferating oligodendrocyte precursor cells (OPCs), mature oligodendrocytes and reactive astrocytes, exhibiting transcriptional commonalities in response to ischemic injury. OPCs and reactive astrocytes are involved in a shared immuno-glial cross talk with stroke-specific myeloid cells. Within the perilesional zone, osteopontin positive myeloid cells accumulate in close proximity to CD44

Identifiants

DOI: 10.1038/s41467-024-50465-z PMID: 39043661

pubmed: 39043661

doi: 10.1038/s41467-024-50465-z

pii: 10.1038/s41467-024-50465-z

doi:

Substances chimiques

Osteopontin 106441-73-0

Spp1 protein, mouse 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

6232

Subventions

Organisme : Österreichische Forschungsförderungsgesellschaft (Austrian Research Promotion Agency)

ID : #898252

Organisme : Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.)

ID : #1R01NS114227-01A1

Organisme : Austrian Science Fund (Fonds zur Förderung der Wissenschaftlichen Forschung)

ID : #T109, #P31085-B26

Informations de copyright

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