Biocatalysis enables the scalable conversion of biobased furans into various furfurylamines.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 Jul 2024
29 Jul 2024
Historique:
received:
25
12
2023
accepted:
17
07
2024
medline:
30
7
2024
pubmed:
30
7
2024
entrez:
29
7
2024
Statut:
epublish
Résumé
Biobased furans have emerged as chemical building blocks for the development of materials because of their diverse scaffolds and as they can be directly prepared from sugars. However, selective, efficient, and cost-effective scalable conversion of biobased furans remains elusive. Here, we report a robust transaminase (TA) from Shimia marina (SMTA) that enables the scalable amination of biobased furanaldehydes with high activity and broad substrate specificity. Crystallographic and mutagenesis analyses provide mechanistic insights and a structural basis for understanding SMTA, which enables a higher substrate conversion. The enzymatic cascade process established in this study allows one-pot synthesis of 2,5-bis(aminomethyl)furan (BAMF) and 5-(aminomethyl)furan-2-carboxylic acid from 5-hydroxymethylfurfural. The biosynthesis of various furfurylamines, including a one-pot cascade reaction for BAMF generation using whole cells, demonstrates their practical application in the pharmaceutical and polymer industries.
Identifiants
pubmed: 39075048
doi: 10.1038/s41467-024-50637-x
pii: 10.1038/s41467-024-50637-x
doi:
Substances chimiques
Furans
0
Transaminases
EC 2.6.1.-
Furaldehyde
DJ1HGI319P
5-hydroxymethylfurfural
70ETD81LF0
Amines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6371Informations de copyright
© 2024. The Author(s).
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