Serratia marcescens ATCC 274 increases production of the red pigment prodigiosin in response to Chi phage infection.
Flagella
Gene expression
Genetic transcription
Phage therapy
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 Jul 2024
31 Jul 2024
Historique:
received:
31
05
2024
accepted:
26
07
2024
medline:
1
8
2024
pubmed:
1
8
2024
entrez:
31
7
2024
Statut:
epublish
Résumé
Serratia marcescens is an opportunistic human pathogen that produces a vibrant red pigment called prodigiosin. Prodigiosin has implications in virulence of S. marcescens and promising clinical applications. We discovered that addition of the virulent flagellotropic bacteriophage χ (Chi) to a culture of S. marcescens stimulates a greater than fivefold overproduction of prodigiosin. Active phage infection is required for the effect, as a χ-resistant strain lacking flagella does not respond to phage presence. Via a reporter fusion assay, we have determined that the addition of a χ-induced S. marcescens cell lysate to an uninfected culture causes a threefold increase in transcription of the pig operon, containing genes essential for pigment biosynthesis. Replacement of the pig promoter with a constitutive promoter abolished the pigmentation increase, indicating that regulatory elements present in the pig promoter likely mediate the phenomenon. We hypothesize that S. marcescens detects the threat of phage-mediated cell death and reacts by producing prodigiosin as a stress response. Our findings are of clinical significance for two main reasons: (i) elucidating complex phage-host interactions is crucial for development of therapeutic phage treatments, and (ii) overproduction of prodigiosin in response to phage could be exploited for its biosynthesis and use as a pharmaceutical.
Identifiants
pubmed: 39085460
doi: 10.1038/s41598-024-68747-3
pii: 10.1038/s41598-024-68747-3
doi:
Substances chimiques
Prodigiosin
OL369FU7CJ
Pigments, Biological
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
17750Subventions
Organisme : Virginia Tech, CeZAP
ID : CeZAP mini-grant
Organisme : National Science Foundation
ID : IOS-2054392
Informations de copyright
© 2024. The Author(s).
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