Novel insights on microbiome dynamics during a gill disease outbreak in farmed rainbow trout (Oncorhynchus mykiss).
Animals
Oncorhynchus mykiss
/ microbiology
Gills
/ microbiology
Fish Diseases
/ microbiology
Microbiota
Flavobacterium
/ genetics
Disease Outbreaks
/ veterinary
RNA, Ribosomal, 16S
/ genetics
Aquaculture
High-Throughput Nucleotide Sequencing
Yersinia ruckeri
/ genetics
Flavobacteriaceae Infections
/ veterinary
Oxytetracycline
/ therapeutic use
Anti-Bacterial Agents
/ pharmacology
Flavobacterium
Gill disease
Microbiome
NGS
Oxytetracycline
Rainbow trout
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 Aug 2024
01 Aug 2024
Historique:
received:
03
04
2024
accepted:
22
07
2024
medline:
2
8
2024
pubmed:
2
8
2024
entrez:
1
8
2024
Statut:
epublish
Résumé
The generic term "Gill disease" refers to a wide range of disorders that affect the gills and severely impact salmonid aquaculture systems worldwide. In rainbow trout freshwater aquaculture, various etiological agents causing gill diseases have been described, particularly Flavobacterium and Amoeba species, but research studies suggest a more complex and multifactorial aetiology. Here, a cohort of rainbow trout affected by gill disease is monitored both through standard laboratory techniques and 16S rRNA Next-Generation Sequencing (NGS) analysis during a natural disease outbreak and subsequent antibiotic treatment with Oxytetracycline. NGS results show a clear clustering of the samples between pre- and post-treatment based on the microbial community of the gills. Interestingly, the three main pathogenic bacteria species in rainbow trout (Yersinia ruckeri, Flavobacterium psychrophilum, and Flavobacterium branchiophilum) appear to be weak descriptors of the diversity between pre-treatment and post-treatment groups. In this study, the dynamics of the gill microbiome during the outbreak and subsequent treatment are far more complex than previously reported in the literature, and environmental factors seem of the utmost importance in determining gill disease. These findings present a potential novel perspective on the diagnosis and management of gill diseases, showing the limitations of conventional laboratory methodologies in elucidating the complexity of this disease in rainbow trout. To the authors' knowledge, this work is the first to describe the microbiome of rainbow trout gills during a natural outbreak and subsequent antibiotic treatment. The results of this study suggest that NGS can play a critical role in the analysis and comprehension of gill pathology. Using NGS in future research is highly recommended to gain deeper insights into such diseases correlating gill's microbiome with other possible cofactors and establish strong prevention guidelines.
Identifiants
pubmed: 39090156
doi: 10.1038/s41598-024-68287-w
pii: 10.1038/s41598-024-68287-w
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Oxytetracycline
X20I9EN955
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
17791Informations de copyright
© 2024. The Author(s).
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